Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-97822 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-97822, RRID:AB_1291807
- Product name
- Rabbit Polyclonal Thyroid receptor-interacting protein 12 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Ubiquitin-dependent and ubiquitin-independent control of subunit stoichiometry in the SWI/SNF complex.
Keppler BR, Archer TK
The Journal of biological chemistry 2010 Nov 12;285(46):35665-74
The Journal of biological chemistry 2010 Nov 12;285(46):35665-74
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Thyroid receptor-interacting protein 12 Antibody [NB100-97822] - Detection of Human TRIP12 on HeLa whole cell lysate using NB100-97822.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Thyroid receptor-interacting protein 12 Antibody [NB100-97822] - Detection of human TRIP12 by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP12 antibody NB100-97822 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Thyroid receptor-interacting protein 12 Antibody [NB100-97822] - Detection of human TRIP12 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIP12 antibody A301-814A (lot A301-814A-2) used for IP at 6 ug per reaction. TRIP12 was also immunoprecipitated by a previous lot of this antibody (lot A301-814A-1). For blotting immunoprecipitated TRIP12, A301-814A was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.