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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [2]
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- Product number
- NB100-655 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-655, RRID:AB_2179725
- Product name
- Rabbit Polyclonal Roquin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Attenuation of AMPK signaling by ROQUIN promotes T follicular helper cell formation.
RC3H1 post-transcriptionally regulates A20 mRNA and modulates the activity of the IKK/NF-κB pathway.
The role of Roquin overexpression in the modulation of signaling during in vitro and ex vivo T-cell activation.
Loss of Roquin induces early death and immune deregulation but not autoimmunity.
Histone deacetylase 9 deficiency protects against effector T cell-mediated systemic autoimmunity.
The ROQUIN family of proteins localizes to stress granules via the ROQ domain and binds target mRNAs.
Ramiscal RR, Parish IA, Lee-Young RS, Babon JJ, Blagih J, Pratama A, Martin J, Hawley N, Cappello JY, Nieto PF, Ellyard JI, Kershaw NJ, Sweet RA, Goodnow CC, Jones RG, Febbraio MA, Vinuesa CG, Athanasopoulos V
eLife 2015 Oct 23;4
eLife 2015 Oct 23;4
RC3H1 post-transcriptionally regulates A20 mRNA and modulates the activity of the IKK/NF-κB pathway.
Murakawa Y, Hinz M, Mothes J, Schuetz A, Uhl M, Wyler E, Yasuda T, Mastrobuoni G, Friedel CC, Dölken L, Kempa S, Schmidt-Supprian M, Blüthgen N, Backofen R, Heinemann U, Wolf J, Scheidereit C, Landthaler M
Nature communications 2015 Jul 14;6:7367
Nature communications 2015 Jul 14;6:7367
The role of Roquin overexpression in the modulation of signaling during in vitro and ex vivo T-cell activation.
Kim HJ, Ji YR, Kim MO, Yu DH, Shin MJ, Yuh HS, Bae KB, Park Sj, Yi JK, Kim NR, Park SJ, Yoon du H, Lee WH, Lee S, Ryoo ZY
Biochemical and biophysical research communications 2012 Jan 6;417(1):280-6
Biochemical and biophysical research communications 2012 Jan 6;417(1):280-6
Loss of Roquin induces early death and immune deregulation but not autoimmunity.
Bertossi A, Aichinger M, Sansonetti P, Lech M, Neff F, Pal M, Wunderlich FT, Anders HJ, Klein L, Schmidt-Supprian M
The Journal of experimental medicine 2011 Aug 29;208(9):1749-56
The Journal of experimental medicine 2011 Aug 29;208(9):1749-56
Histone deacetylase 9 deficiency protects against effector T cell-mediated systemic autoimmunity.
Yan K, Cao Q, Reilly CM, Young NL, Garcia BA, Mishra N
The Journal of biological chemistry 2011 Aug 19;286(33):28833-43
The Journal of biological chemistry 2011 Aug 19;286(33):28833-43
The ROQUIN family of proteins localizes to stress granules via the ROQ domain and binds target mRNAs.
Athanasopoulos V, Barker A, Yu D, Tan AH, Srivastava M, Contreras N, Wang J, Lam KP, Brown SH, Goodnow CC, Dixon NE, Leedman PJ, Saint R, Vinuesa CG
The FEBS journal 2010 May;277(9):2109-27
The FEBS journal 2010 May;277(9):2109-27
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Roquin Antibody [NB100-655] - Detection of human Roquin on HeLa whole cell lysate using NB100-655. Roquin was also immunoprecipitated using NB100-656 at 3 ug/mg lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Roquin Antibody [NB100-655] - Detection of Mouse Roquin by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-655 used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
