A300-894A
antibody from Invitrogen Antibodies
Targeting: COP1
CFAP78, FAP78, FLJ10416, RFWD2, RNF200
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Other assay [1]
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Validation data
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- Product number
- A300-894A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COP1/RFWD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse COP1/RFWD2 by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2, U2OS, K-562, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COP1/RFWD2 antibody (Product # A300-894A; lot A300-894A-3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse COP1/RFWD2 by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2, U2OS, K-562, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COP1/RFWD2 antibody (Product # A300-894A; lot A300-894A-3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human COP1/RFWD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-COP1/RFWD2 antibody A300-894A (lot A300-894A-2) used for IP at 6 µg per reaction. COP1/RFWD2 was also immunoprecipitated by rabbit anti-COP1/RFWD2 antibody A300-895A. For blotting immunoprecipitated COP1/RFWD2, A300-894A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse COP1/RFWD2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COP1/RFWD2 antibody A300-894A (lot A300-894A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human COP1/RFWD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COP1/RFWD2 antibody (Product # A300-894A; lot A300-894A-3) used for IP at 6 µg per reaction. COP1/RFWD2 was also immunoprecipitated by a previous lot of this antibody (lot A300-894A-2). For blotting immunoprecipitated COP1/RFWD2, Product # A300-894A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.