- PA5-28161 - Invitrogen Antibodies ADAM10 antibody

Submitted references Milk Exosomes Prevent Intestinal Inflammation in a Genetic Mouse Model of Ulcerative Colitis: A Pilot Experiment.

Stremmel W, Weiskirchen R, Melnik BC
Inflammatory intestinal diseases 2020 Aug;5(3):117-123

ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways.

Li W, Wang D, Sun X, Zhang Y, Wang L, Suo J
International journal of molecular medicine 2019 Feb;43(2):914-926

Iron-Overload triggers ADAM-17 mediated inflammation in Severe Alcoholic Hepatitis.

Maras JS, Das S, Sharma S, Sukriti S, Kumar J, Vyas AK, Kumar D, Bhat A, Yadav G, Choudhary MC, Sharma S, Kumar G, Bihari C, Trehanpati N, Maiwall R, Sarin SK
Scientific reports 2018 Jul 6;8(1):10264

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using ADAM10 Polyclonal Antibody (Product # PA5-28161). Sample (30 µg of whole cell lysate). Lane A: THP-1. 7.5% SDS PAGE. ADAM10 Polyclonal Antibody (Product # PA5-28161) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using ADAM10 Polyclonal Antibody (Product # PA5-28161). Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 Polyclonal Antibody (Product # PA5-28161) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human lung Papillary adenocarcinoma, using ADAM10 (Product # PA5-28161) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Increased CD163 and ADAM17 expression is associated to iron-overload in SAH. ( A ) Workflow and summary of the experiments performed and number of samples used in target determination, and validation experiments including the In-vivo and In-vitro analysis. ( B ) Venn-diagram for unique and shared genes (p < 0.05, fold change >1.5) in the liver and PBMC transcriptome of Group A: SAH-IO.16 shared genes in the liver and PBMC were associated to cellular iron ion homeostasis, cytokine receptor binding and notch signaling. ( C ) Venn-diagram for unique and shared genes identified in the univariate and multivariate projection analysis for the liver and PBMC transcriptome of Group A: SAH-IO documenting CD163, ADAM17 and TMED7 significantly associated to systemic iron overload. ( D ) Immuno-histochemistry (IHC) for iron deposition (Perl's-staining) and expression of ADAM17, CD163, ADAM10 in Group A: SAH-IO (n = 5) vs. Group B: SAH-NIO (n = 5). [For all IHC analysis relative quantization of positively stained cells are expressed as mean number of positive cells/10 high power field (40x)]. ( E ) Perl's staining and expression of CD68, ADAM17, CD163 and ADAM10 in Group A: SAH-IO (n = 5) vs. AC (n = 5). ( F ) Perl's staining, and expression of ADAM17, CD163 and ADAM10 in Thalassemia (n = 3) patients compared to Group A and B.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Immunohistochemical analysis showing the expression of ADAM8, ADAM9, ADAM10, ADAM12 and ADAM17 in adjacent non-cancerous gastric tissues, gastric cancer tissues, positive metastatic lymph node tissues, and negative metastatic lymph node tissues. Scale bars, 50 u M. ADAM, disintegrin and metalloproteinase domain-containing protein.

PA5-28161

Antibody data