PA1-973

Cho KI, Patil H, Senda E, Wang J, Yi H, Qiu S, Yoon D, Yu M, Orry A, Peachey NS, Ferreira PA
The Journal of biological chemistry 2014 Feb 21;289(8):4600-25

Glucosamine induces cell death via proteasome inhibition in human ALVA41 prostate cancer cell.

Liu BQ, Meng X, Li C, Gao YY, Li N, Niu XF, Guan Y, Wang HQ
Experimental & molecular medicine 2011 Sep 30;43(9):487-93

Persistent hijacking of brain proteasomes in HIV-associated dementia.

Nguyen TP, Soukup VM, Gelman BB
The American journal of pathology 2010 Feb;176(2):893-902

The cyclophilin-like domain of Ran-binding protein-2 modulates selectively the activity of the ubiquitin-proteasome system and protein biogenesis.

Yi H, Friedman JL, Ferreira PA
The Journal of biological chemistry 2007 Nov 30;282(48):34770-8

Reduced ubiquitin C-terminal hydrolase-1 expression levels in dementia with Lewy bodies.

Barrachina M, Castaño E, Dalfó E, Maes T, Buesa C, Ferrer I
Neurobiology of disease 2006 May;22(2):265-73

Neuropathology and pathogenesis of encephalitis following amyloid-beta immunization in Alzheimer's disease.

Ferrer I, Boada Rovira M, Sánchez Guerra ML, Rey MJ, Costa-Jussá F
Brain pathology (Zurich, Switzerland) 2004 Jan;14(1):11-20

Neuropathology and pathogenesis of encephalitis following amyloid-beta immunization in Alzheimer's disease.

Ferrer I, Boada Rovira M, Sánchez Guerra ML, Rey MJ, Costa-Jussá F
Brain pathology (Zurich, Switzerland) 2004 Jan;14(1):11-20

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µglysate) of MCF7 (Lane 1), PC-3 (Lane 2), Hep G2 (Lane 3), SK-OV-3 (Lane 4), HeLa (Lane 5), COS-7 (Lane 6), Jurkat (Lane 7), A-431 (Lane 8) and tissue extracts (30 µglysate) of Mouse Testis (Lane 9) and Rat Testis (Lane 10). The blot was probed with Anti-PSMD1 Rabbit Polyclonal Antibody (Product # PA1-973, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 106 kDa band corresponding to PSMD1 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with overnight wet transfer system. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of PSMD1 was done on MCF7 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with PSMD1 Rabbit Polyclonal Antibody (PA1973, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL