MA5-34674

antibody from Invitrogen Antibodies

Targeting: NPHS2 PDCN, SRN1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34674

Provider

Invitrogen Antibodies

Product name

Podocin Recombinant Rabbit Monoclonal Antibody (JB51-33)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: Rat kidney tissue, human kidney tissue, mouse kidney tissue, 293T.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

JB51-33

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NPHS2 in rat kidney (1) and human kidney (2) tissue lysate. Samples were incubated with NPHS2 monoclonal antibody (Product # MA5-34674), at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Podocin Recombinant Rabbit Monoclonal Antibody (JB51-33) (Product # MA5-34674) and a ~42 kDa band corresponding to Nphs2 was observed only in mouse kidney tissue. Whole cell extracts (30 µg lysate) of Mouse Kidney (Lane 1), Rat Kidney (Lane 2), Mouse Liver (Lane 3), Rat Liver (Lane 4), Mouse Skeletal Muscle (Lane 5), Rat Skeletal Muscle (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NPHS2 in paraffin-embedded human kidney tissue. Samples were incubated with NPHS2 monoclonal antibody (Product # MA5-34674), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NPHS2 in paraffin-embedded rat kidney tissue. Samples were incubated with NPHS2 monoclonal antibody (Product # MA5-34674), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NPHS2 in paraffin-embedded mouse kidney tissue. Samples were incubated with NPHS2 monoclonal antibody (Product # MA5-34674), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NPHS2 in paraffin-embedded human kidney tissue. Samples were incubated with NPHS2 monoclonal antibody (Product # MA5-34674), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of NPHS2 in 293T cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with NPHS2 monoclonal antibody (Product # MA5-34674) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.