20384-1-AP

antibody from Invitrogen Antibodies

Targeting: NPHS2 PDCN, SRN1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

20384-1-AP

Provider

Invitrogen Antibodies

Product name

NPHS2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Applications Reported: This MBSA43 antibody has been reported for use in flow cytometric analysis.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.22 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse colon tissue were subjected to SDS PAGE followed by western blot with 20384-1-AP (NPHS2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 20384-1-AP (NPHS2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse colon tissue were subjected to SDS PAGE followed by western blot with 20384-1-AP (NPHS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

The podocin antibody 20384-1-AP recognized the zebrafish pronephric glomeruli in zebrafish embryos (72 hour-old fish). IF result from Dr. Weibin Zhou.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 20384-1-AP (NPHS2 antibody) at dilution of 1:500 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 20384-1-AP (NPHS2 antibody) at dilution of 1:500 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).