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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-22008 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDE6D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: Molt-4, mouse eye.
- Concentration
- 1.73 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDE6D using 30 µg of MOLT4 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a PDE6D polyclonal antibody (Product # PA5-22008) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDE6D was performed by separating 50 µg of mouse tissue extract by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a PDE6D Polyclonal Antibody (Product # PA5-22008) at a dilution of 1:15000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PDE6D Polyclonal Antibody (Product # PA5-22008). Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with PDE6D Polyclonal Antibody (Product # PA5-22008) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PDE6D in methanol-fixed HeLa cells using a PDE6D polyclonal antibody (Product # PA5-22008) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using PDE6D antibody (Product # PA5-22008) at 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PDE6D (Product # PA5-22008) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
