NB120-2737

antibody from Novus Biologicals

Targeting: TOR1AIP1 FLJ13142, LAP1, LAP1B, LAP1C

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB120-2737

Provider

Novus Biologicals

Proper citation

Novus Cat#NB120-2737, RRID:AB_2056883

Product name

Mouse Monoclonal LAP1 Antibody

Antibody type

Monoclonal

Description

PEG purified. Detects lamin associated polypeptide (LAP) 1A, 1B & 1C from rat tissues. This does not cross-react with LAP 2.This detects 3 distinct proteins of molecular weights 75, 68 and 55 kDa representing LAP 1A, 1B and 1C, respectively in rat liver nuclear envelope fractions. Immunofluorescence staining of LAP 1 in rat liver with NB120-2737 results in exclusive labeling of the nuclear periphery and exhibits co-localization with lamin staining.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

200uL

Concentration

13.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: LAP1 Antibody (RL13) [NB120-2737] - Immunohistochemistry was performed on rat lymph node tissue. To expose target protein, antigen was retreived. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LAP1 mouse monoclonal antibody at a dilution of 1:50 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: LAP1 Antibody (RL13) [NB120-2737] - Immunohistochemistry was performed on rat colon tissue. To expose target protein, antigen was retreived. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LAP1 mouse monoclonal antibody at a dilution of 1:20 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: LAP1 Antibody (RL13) [NB120-2737] - Immunohistochemistry was performed on rat breast tissue. To expose target protein, antigen was retreived. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LAP1 mouse monoclonal antibody at a dilution of 1:50 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.