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antibody from Novus Biologicals
Targeting: ABCB1
ABC20, CD243, CLCS, GP170, MDR1, P-gp, PGY1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-80870 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-80870, RRID:AB_1109789
- Product name
- Rabbit Polyclonal MDR1/ABCB1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Amino acids 262-277 of human ABCB1 protein.
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Submitted references Hypoxia-tropic Protein Nanocages for Modulation of Tumor- and Chemotherapy-Associated Hypoxia.
Huang X, Zhuang J, Chung SW, Huang B, Halpert G, Negron K, Sun X, Yang J, Oh Y, Hwang PM, Hanes J, Suk JS
ACS nano 2019 Jan 22;13(1):236-247
ACS nano 2019 Jan 22;13(1):236-247
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MDR1/ABCB1 Antibody [NB100-80870] - Analyis of anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:600, followed by Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG at 1:10000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NB100-80870] - Staining of human kidney tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
