PAB9962

antibody from Abnova Corporation

Targeting: ABCB1 ABC20, CD243, CLCS, GP170, MDR1, P-gp, PGY1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB9962

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB9962, RRID:AB_1672063

Product name

ABCB1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of ABCB1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Modulation of MDR1 gene expression in multidrug resistant MCF7 cells by low concentrations of small interfering RNAs.

Stierlé V, Laigle A, Jollès B
Biochemical pharmacology 2005 Nov 15;70(10):1424-30

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The remarkable transport mechanism of P-glycoprotein: a multidrug transporter.

Al-Shawi MK, Omote H
Journal of bioenergetics and biomembranes 2005 Dec;37(6):489-96

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Epigenetic changes to the MDR1 locus in response to chemotherapeutic drugs.

Baker EK, Johnstone RW, Zalcberg JR, El-Osta A
Oncogene 2005 Dec 1;24(54):8061-75

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using ABCB1 polyclonal antibody (Cat # PAB9962) shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1.The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose.The membrane was probed with the primary antibody diluted to 1 : 600, followed by Peroxidase Conjugated Affinity Purified Anti-Rabbit IgG at 1 : 10,000.Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.