Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-24852 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARPC5 Recombinant Rabbit Monoclonal Antibody (1H9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 1H9
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARPC5 in Lane 1: MCF7 whole cell lysate, Lane 2: SK-BR-3 whole cell lysate. Samples were incubated with ARPC5 monoclonal antibody (Product # MA5-24852) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARPC5 in Lane 1: MCF7 whole cell lysate, Lane 2: SK-BR-3 whole cell lysate. Samples were incubated with ARPC5 monoclonal antibody (Product # MA5-24852) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ARPC5 in N2A cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with ARPC5 monoclonal antibody (Product # MA5-24852).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ARPC5 in paraffin-embedded mouse lung tissue. Samples were incubated with ARPC5 monoclonal antibody (Product # MA5-24852) followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ARPC5 in paraffin-embedded human spleen tissue. Samples were incubated with ARPC5 monoclonal antibody (Product # MA5-24852) followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of ARPC5 in SH-SY-5Y cells (blue), unlabeled control (cells without incubation with primary antibody; red). Samples were incubated with ARPC5 monoclonal antibody (Product # MA5-24852) at a dilution of 1:50 followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.