Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- OMA1-06020 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Smoothelin Monoclonal Antibody (R4A)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- OMA1-06020 detects smoothelin from human, canine, feline, porcine, chicken and monkey tissues. This antibody detects both the visceral and vascular smoothelin isoforms. Does not cross react with rat samples.
- Antibody clone number
- R4A
- Concentration
- 1 mg/mL
Submitted references Palmitate induces glycosylation of cyclooxygenase-2 in primary human vascular smooth muscle cells.
Exercise training attenuates coronary smooth muscle phenotypic modulation and nuclear Ca2+ signaling.
Raman P, Madhavpeddi L, Gonzales RJ
American journal of physiology. Cell physiology 2018 May 1;314(5):C545-C553
American journal of physiology. Cell physiology 2018 May 1;314(5):C545-C553
Exercise training attenuates coronary smooth muscle phenotypic modulation and nuclear Ca2+ signaling.
Wamhoff BR, Bowles DK, Dietz NJ, Hu Q, Sturek M
American journal of physiology. Heart and circulatory physiology 2002 Dec;283(6):H2397-410
American journal of physiology. Heart and circulatory physiology 2002 Dec;283(6):H2397-410
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Smoothelin Monoclonal Antibody (R4A) (Product # OMA1-06020) and a 100kDa band corresponding to Smoothelin was observed across cell lines and tissues tested . Nuclear enriched extracts (30 µg lysate) of PC-3 (Lane 1), HeLa (Lane 2), Mouse Uterus (Lane 3), Mouse Brain (Lane 4) and Mouse Lung (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:5000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry on frozen section of chicken gizzard striated muscle stained with Smoothelin monoclonal antibody (Product # OMA1-06020).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry on frozen section of chicken gizzard striated muscle stained with Smoothelin monoclonal antibody (Product # OMA1-06020).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry on paraffin section of human smooth muscle stained with Smoothelin monoclonal antibody (Product # OMA1-06020).