Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-62481 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Smoothelin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LAGLDEGALR KLLEVTADLA ERRRIRSAIR ELQRQELERE EEALASKRFR AERQDNKENW LHSQQREAEQ RAALARLAGQ LESMNDVEEL TALLR
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Smoothelin in human cell lines PC-3 and HeLa using a Smoothelin Polyclonal Antibody (Product # PA5-62481). Corresponding SMTN RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Smoothelin in human cell line U-2 OS shows positivity in actin filaments & nucleus but excluded from the nucleoli. Samples were probed using a Smoothelin Polyclonal Antibody (Product # PA5-62481).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Smoothelin in human smooth muscle shows moderate cytoplasmic and nuclear positivity in smooth muscle cells. Samples were probed using a Smoothelin Polyclonal Antibody (Product # PA5-62481).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Smoothelin in human smooth muscle and tonsil tissues using Smoothelin Polyclonal Antibody (Product # PA5-62481). Corresponding SMTN RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Smoothelin in human tonsil using Smoothelin Polyclonal Antibody (Product # PA5-62481) shows low expression as expected.