AP00597PU-N
antibody from Acris Antibodies GmbH
Targeting: PRG4
bG174L6.2, CACP, FLJ32635, HAPO, JCAP, MSF, SZP
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- AP00597PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP00597PU-N, RRID:AB_1006569
- Product name
- anti Proteoglycan 4 (1356-1374)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponds to amino acid residues 1356-1374 of human Lubricin.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1 shows a Western blot with human recombinant Lubricin (280kDa band) using AP00597PU-N.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Lubricin was performed by loading 3 µg of non-reduced human synovial fluid per well onto a SDS-PAGE gel. Proteins were transferred to a membrane. The membrane was probed with a Polyclonal Lubricin Antibody (Cat.-No AP00597PU-N) at a concentration of 2 µg/ml diluted in the antibody diluent provided in the Fast Western Blot Kit, followed by the optimized HRP reagent from the same kit (diluted by adding 150 µl of HRP reagent to 10 ml of reagent diluent). Chemiluminescent detection was performed using SuperSignal West Dura.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot with Human recombinant Lubricin (280kDa band) using Lubricin Antibody Cat.-No AP00597PU-N.