Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB120-7046 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-7046, RRID:AB_789363
- Product name
- Rabbit Polyclonal Collagen V Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- Reactivity
- Human, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Submitted references The Role of Titanium Surface Microtopography on Adhesion, Proliferation, Transformation, and Matrix Deposition of Corneal Cells.
Zhou C, Lei F, Chodosh J, Paschalis EI
Investigative ophthalmology & visual science 2016 Apr 1;57(4):1927-38
Investigative ophthalmology & visual science 2016 Apr 1;57(4):1927-38
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Collagen V Antibody [NB120-7046] - Analysis using the Biotin conjugate of NB120-7046. collagen V antibody 1:200, 45 min RT showed strong staining in FFPE sections of human lung (left) with strong staining within alveoli, vessels, and in connective tissue spaces; and placenta (right) with strong staining observed in stromal and connective tissue spaces and vessel walls. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval.