NB120-7046

antibody from Novus Biologicals

Targeting: COL5A1

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB120-7046

Provider

Novus Biologicals

Proper citation

Novus Cat#NB120-7046, RRID:AB_789363

Product name

Rabbit Polyclonal Collagen V Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Reactivity

Human, Bovine

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.

References

Submitted references

The Role of Titanium Surface Microtopography on Adhesion, Proliferation, Transformation, and Matrix Deposition of Corneal Cells.

Zhou C, Lei F, Chodosh J, Paschalis EI
Investigative ophthalmology & visual science 2016 Apr 1;57(4):1927-38

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Collagen V Antibody [NB120-7046] - Analysis using the Biotin conjugate of NB120-7046. collagen V antibody 1:200, 45 min RT showed strong staining in FFPE sections of human lung (left) with strong staining within alveoli, vessels, and in connective tissue spaces; and placenta (right) with strong staining observed in stromal and connective tissue spaces and vessel walls. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval.