17056-1-AP

antibody from Invitrogen Antibodies

Targeting: UBE2F NCE2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

17056-1-AP

Provider

Invitrogen Antibodies

Product name

UBE2F Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MLTLASKLK RDDGLKGSRT AATASDSTRR VSVRDKLLVK EVAELEANLP CTCKVHFPDP NKLHCFQLTV TPDEGYYQGG KFQFETEVPD AYNMVPPKVK CLTKIWHPNI TETGEICLSL LREHSIDGTG WAPTRTLKDV VWGLNSLFTD LLNFDDPLNI EAAEHHLRDK EDFRNKVDDY IKRYAR (1-185 aa encoded by B C010549)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 17056-1-AP (UBE2F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17056-1-AP (UBE2F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed NIH/3T3 cells using 17056-1-AP (UBE2F antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin using 17056-1-AP (UBE2F antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin using 17056-1-AP (UBE2F antibody) at dilution of 1:100 (under 40x lens).