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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX54521 - Provider product page
- Provider
- GeneTex
- Product name
- Huntingtin (neoepitope 552) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of endogenous Htt lysates with or without different caspase activity (lanes 1-5) and overexpressed recombinant Htt fragment lysates (lanes 6-9) (20 ug per well) using pan-Huntingtin antibody (top, N-terminal HTT) or Huntingtin (neoepitope 552) antibody at a dilution of 1:500 (bottom, neoHtt552).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sandwich ELISA was performed with a Huntingtin monoclonal antibody and Huntingtin (neoepitope 552) antibody to determine the antigen concentration of the Huntingtin cleavage products. The curve represents a dose response for neo552 in 293T cells overexpressing the Htt construct.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of Caspase cleaved Htt (green) in 293T cells transfected with an Htt23Q (left) and Htt148Q (right) using Huntingtin (neoepitope 552) antibody at a dilution of 1:50 (green). Nuclei (blue) were stained with Hoechst dye.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC analysis of Cortex tissue from transgenic HD mice of the YAC128 line using Huntingtin (neoepitope 552) antibody at a dilution of 1:50 (green). Tissues were counterstained with nuclei stain (blue).
