LS-C18912

antibody from LSBio

Targeting: UBA1 A1S9T, CFAP124, GXP1, POC20, UBE1, UBE1X

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C18912

Provider

LSBio

Product name

UBA1 / UBE1 Antibody LS-C18912

Antibody type

Polyclonal

Description

Protein A purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store vial at 4°C prior to restoration. Reconstitute with 0.1 ml of deionized water or equivalent. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Ubiquitin Activating Enzyme (E1) Antibody - Western Blot. Western blot of purified anti-Ubiquitin Activating Enzyme (E1) antibody shows detection of a band at ~118 kD corres-ponding to UBE1 (lane 1 800 nm channel). Approximately 35 ug of an A431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX10 ( for 45 min at room temperature. Molecular weight markers are shown in lane 2 (700 nm channel). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Ubiquitin Activating Enzyme Antibody - Immunohistochemistry. Affinity Purified anti-Ubiquitin Activating Enzyme antibody was used at a 10 ug/ml to detect UBE1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (islet and exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis, thymus, tonsil, and uterus (glandular, stroma). In many cells a punctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining. This image shows UBE1 staining of human lung tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.