TA319259

antibody from OriGene

Targeting: UBA1 A1S9T, CFAP124, GXP1, POC20, UBE1, UBE1X

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319259

Provider

OriGene

Product name

Rabbit polyclonal Ubiquitin Activating Enzyme E1 antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal Ubiquitin Activating Enzyme E1 antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

UBA1

Isotype

IgG

Antibody clone number

NULL

Vial size

500 µg

Concentration

5.0 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB using anti-Ubiquitin Activating Enzyme (E1) antibody shows detection of a band at ~118 kDa corresponding to UBE1 (lane 1800 nm channel). Primary antibody was used at a 1:1,000 dilution. Secondry antibody was a 1:10,000 dilution of IRDye„?800 conjugated Gt-a-Rabbit IgG [H&L]. Molecular weight markers are shown in lane 2 (700 nm channel).

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-Ubiquitin Activating Enzyme antibody was used at a 10 ug/ml to detect UBE1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (islet and exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis, thymus, tonsil, and uterus (glandular, stroma).

Validation comment

IHC