Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA319259 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal Ubiquitin Activating Enzyme E1 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal Ubiquitin Activating Enzyme E1 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- UBA1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 500 µg
- Concentration
- 5.0 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using anti-Ubiquitin Activating Enzyme (E1) antibody shows detection of a band at ~118 kDa corresponding to UBE1 (lane 1800 nm channel). Primary antibody was used at a 1:1,000 dilution. Secondry antibody was a 1:10,000 dilution of IRDye?800 conjugated Gt-a-Rabbit IgG [H&L]. Molecular weight markers are shown in lane 2 (700 nm channel).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-Ubiquitin Activating Enzyme antibody was used at a 10 ug/ml to detect UBE1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (islet and exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis, thymus, tonsil, and uterus (glandular, stroma).
- Validation comment
- IHC