Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 679404 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#679404, RRID:AB_2566181
- Product name
- Alexa Fluor® 594 anti-E1 Ubiquitin Activating Enzyme
- Antibody type
- Monoclonal
- Antigen
- Mixture of human and mouse E1 protein.
- Description
- The proteasomal pathway of protein degradation involves two discrete steps: ubiquitination and degradation. Aside from proteasomal degradation, ubiquitination also plays an important role in transcriptional regulation, protein trafficking including endocytosis and lysosomal targeting, and the activation of kinases involved in signaling processes. Ubiquitination is carried out in three main steps: activation, conjugation, and ligation. E1 Ubiquitin Activating Enzyme (UBE1) initiates the cascade of conjugation by activating their respective modifiers. It is highly conserved in human, yeast, and plants. The gene for E1 Ubiquitin activating enzyme complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- 2G2.3.5
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- HeLa cells were fixed with 2% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for give minutes, and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 2 µg/ml Alexa Fluor® 594 conjugated (red) E1 Ubiquitin Activating Enzyme Antibody (clone 2G2.3.5) in a blocking buffer overnight at 4°C. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
- Conjugate
- Yellow dye