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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PAB1772 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1772, RRID:AB_1238136
- Product name
- UBA1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of UBA1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Complementation by a cloned human ubiquitin-activating enzyme E1 of the S-phase-arrested mouse FM3A cell mutant with thermolabile E1.
Human ubiquitin-activating enzyme (E1): compensation for heat-labile mouse E1 and its gene localization on the X chromosome.
Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1.
Ayusawa D, Kaneda S, Itoh Y, Yasuda H, Murakami Y, Sugasawa K, Hanaoka F, Seno T
Cell structure and function 1992 Apr;17(2):113-22
Cell structure and function 1992 Apr;17(2):113-22
Human ubiquitin-activating enzyme (E1): compensation for heat-labile mouse E1 and its gene localization on the X chromosome.
Kudo M, Sugasawa K, Hori T, Enomoto T, Hanaoka F, Ui M
Experimental cell research 1991 Jan;192(1):110-7
Experimental cell research 1991 Jan;192(1):110-7
Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1.
Handley PM, Mueckler M, Siegel NR, Ciechanover A, Schwartz AL
Proceedings of the National Academy of Sciences of the United States of America 1991 Jan 1;88(1):258-62
Proceedings of the National Academy of Sciences of the United States of America 1991 Jan 1;88(1):258-62
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The UBA1 polyclonal antibody (Cat # PAB1772) is used in Western blot to detect UBA1 in HL-60 cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBA1 polyclonal antibody (Cat # PAB1772) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with UBA1 polyclonal antibody (Cat # PAB1772) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
