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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB11287 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11287, RRID:AB_1716718
- Product name
- UBA1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against full length recombinant UBA1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Screening of Hsp105alpha-binding proteins using yeast and bacterial two-hybrid systems.
Large-scale characterization of HeLa cell nuclear phosphoproteins.
A non-proteolytic role for ubiquitin in Tat-mediated transactivation of the HIV-1 promoter.
Saito Y, Doi K, Yamagishi N, Ishihara K, Hatayama T
Biochemical and biophysical research communications 2004 Feb 6;314(2):396-402
Biochemical and biophysical research communications 2004 Feb 6;314(2):396-402
Large-scale characterization of HeLa cell nuclear phosphoproteins.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
A non-proteolytic role for ubiquitin in Tat-mediated transactivation of the HIV-1 promoter.
Brès V, Kiernan RE, Linares LK, Chable-Bessia C, Plechakova O, Tréand C, Emiliani S, Peloponese JM, Jeang KT, Coux O, Scheffner M, Benkirane M
Nature cell biology 2003 Aug;5(8):754-61
Nature cell biology 2003 Aug;5(8):754-61
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using UBA1 polyclonal antibody (Cat # PAB11287) shows detection of a band at ~118 KDa corres-ponding to UBA1 (Lane 1 800 nm channel).Approximately 35 ug of an A-431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1 : 1,000.Incubation was for 2 h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™ 800 conjugated Gt-a-Rabbit IgG [H&L]MX10 for 45 min at room temperature.Molecular weight markers are shown in lane 2 (700 nm channel).IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of UBA1 polyclonal antibody (Cat # PAB11287) was used at a 10 ug/mL to detect UBA1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (isletand exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis,thymus, tonsil, and uterus (glandular, stroma).In many cells a punctate nuclear staining was observed.Other cells showedboth cytoplasmic and nuclear staining.This image shows UBA1 staining of human lung tissue.Tissue was formalin-fixed and paraffin embedded.Personal Communi-cation, TinaRoush, Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
