NBP2-67816

antibody from Novus Biologicals

Targeting: UBA1 A1S9T, CFAP124, GXP1, POC20, UBE1, UBE1X

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-67816

Provider

Novus Biologicals

Product name

Rabbit Monoclonal Ubiquitin-activating Enzyme/UBE1 Antibody

Antibody type

Monoclonal

Description

Protein A purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Analysis of UBA1 on mouse liver lysates using anti-UBA1 antibody at 1:5000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Whole spinal cord extracts from P8 mice. UBE1 band can be seen at 130 kDa. Antibody at 1:500. Image from verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Human skin fibroblast cell lysate, 5 ug total protein. Primary antibody at 1:500. Image from verified customer review.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Analysis of paraffin-embedded human kidney tissue using anti-UBA1 antibody. Counter stained with hematoxylin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Analysis of paraffin-embedded mouse ovary tissue using anti-UBA1 antibody. Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Analysis of K562 cells with UBA1 antibody at 1:50 (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.