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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- 66544-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TWIST2 Monoclonal Antibody (1B12D11)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: MEEGSSSPV SPVDSLGTSE EELERQPKRF GRKRRYSKKS SEDGSPTPGK RGKKGSPSAQ SFEELQSQRI LANVRERQRT QSLNEAFAAL RKIIPTLPSD KLSKIQTLKL AARYIDFLYQ VLQSDEMDNK MTSCSYVAHE RLSYAFSVWR MEGAWSMSAS H (1-160 aa encoded by BC033168)
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1B12D11
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various cells were subjected to SDS PAGE followed by western blot with 66544-1-IG (TWIST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 66544-1-IG (TWIST2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66544-1-IG ( TWIST2 antibody) at dilution of 1:500 (under 40x lens).
