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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-21474 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NDUFA10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse kidney.
- Concentration
- 0.82 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NDUFA10 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a NDUFA10 Polyclonal Antibody (Product # PA5-21474) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NDUFA10 Polyclonal Antibody (Product # PA5-21474). Sample (30 µg of whole cell lysate). A: H1299. B: Raji. 10% SDS PAGE. NDUFA10 Polyclonal Antibody (Product # PA5-21474) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NDUFA10 Polyclonal Antibody detects NDUFA10 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: NDUFA10 protein stained by NDUFA10 Polyclonal Antibody (Product # PA5-21474) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon , using NDUFA10 (Product # PA5-21474) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
