NB110-39113

Baumann T, Dunkel A, Schmid C, Schmitt S, Hiltensperger M, Lohr K, Laketa V, Donakonda S, Ahting U, Lorenz-Depiereux B, Heil JE, Schredelseker J, Simeoni L, Fecher C, Körber N, Bauer T, Hüser N, Hartmann D, Laschinger M, Eyerich K, Eyerich S, Anton M, Streeter M, Wang T, Schraven B, Spiegel D, Assaad F, Misgeld T, Zischka H, Murray PJ, Heine A, Heikenwälder M, Korn T, Dawid C, Hofmann T, Knolle PA, Höchst B
Nature immunology 2020 May;21(5):555-566

A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism.

Ho JJD, Balukoff NC, Theodoridis PR, Wang M, Krieger JR, Schatz JH, Lee S
Nature communications 2020 May 29;11(1):2677

Autophagy is critical for group 2 innate lymphoid cell metabolic homeostasis and effector function.

Galle-Treger L, Hurrell BP, Lewis G, Howard E, Jahani PS, Banie H, Razani B, Soroosh P, Akbari O
The Journal of allergy and clinical immunology 2020 Feb;145(2):502-517.e5

Effect of high glucose condition on glucose metabolism in primary astrocytes.

Staricha K, Meyers N, Garvin J, Liu Q, Rarick K, Harder D, Cohen S
Brain research 2020 Apr 1;1732:146702

Differential regulation of AMP-activated protein kinase in healthy and cancer cells explains why V-ATPase inhibition selectively kills cancer cells.

Bartel K, Müller R, von Schwarzenberg K
The Journal of biological chemistry 2019 Nov 15;294(46):17239-17248

Local suture ligation-assisted percutaneous sclerotherapy for Kasabach-Merritt phenomenon-associated kaposiform haemangioendothelioma.

Li X, Wen MZ, Su LX, Yang XT, Han YF, Fan XD
Oncology letters 2019 Jan;17(1):981-989

A Potential Role for GSK3β in Glucose-Driven Intrauterine Catch-Up Growth in Maternal Obesity.

Appel S, Grothe J, Storck S, Janoschek R, Bae-Gartz I, Wohlfarth M, Handwerk M, Hucklenbruch-Rother E, Gellhaus A, Dötsch J
Endocrinology 2019 Feb 1;160(2):377-386

Pioglitazone improves working memory performance when administered in chronic TBI.

McGuire JL, Correll EA, Lowery AC, Rhame K, Anwar FN, McCullumsmith RE, Ngwenya LB
Neurobiology of disease 2019 Dec;132:104611

Unique pattern of neutrophil migration and function during tumor progression.

Patel S, Fu S, Mastio J, Dominguez GA, Purohit A, Kossenkov A, Lin C, Alicea-Torres K, Sehgal M, Nefedova Y, Zhou J, Languino LR, Clendenin C, Vonderheide RH, Mulligan C, Nam B, Hockstein N, Masters G, Guarino M, Schug ZT, Altieri DC, Gabrilovich DI
Nature immunology 2018 Nov;19(11):1236-1247

Dietary Fiber Confers Protection against Flu by Shaping Ly6c^- Patrolling Monocyte Hematopoiesis and CD8⁺ T Cell Metabolism.

Trompette A, Gollwitzer ES, Pattaroni C, Lopez-Mejia IC, Riva E, Pernot J, Ubags N, Fajas L, Nicod LP, Marsland BJ
Immunity 2018 May 15;48(5):992-1005.e8

Application of an acellular dermal matrix to a rabbit model of oral mucosal defects.

Xu X, Cui N, Wang E
Experimental and therapeutic medicine 2018 Mar;15(3):2450-2456

Fatty acid metabolism complements glycolysis in the selective regulatory T cell expansion during tumor growth.

Pacella I, Procaccini C, Focaccetti C, Miacci S, Timperi E, Faicchia D, Severa M, Rizzo F, Coccia EM, Bonacina F, Mitro N, Norata GD, Rossetti G, Ranzani V, Pagani M, Giorda E, Wei Y, Matarese G, Barnaba V, Piconese S
Proceedings of the National Academy of Sciences of the United States of America 2018 Jul 10;115(28):E6546-E6555

Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B.

Ho JJD, Balukoff NC, Cervantes G, Malcolm PD, Krieger JR, Lee S
Cell reports 2018 Jan 2;22(1):17-26

Prion protein modulates glucose homeostasis by altering intracellular iron.

Ashok A, Singh N
Scientific reports 2018 Apr 26;8(1):6556

Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis.

Kishore M, Cheung KCP, Fu H, Bonacina F, Wang G, Coe D, Ward EJ, Colamatteo A, Jangani M, Baragetti A, Matarese G, Smith DM, Haas R, Mauro C, Wraith DC, Okkenhaug K, Catapano AL, De Rosa V, Norata GD, Marelli-Berg FM
Immunity 2018 Apr 17;48(4):831-832

Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses.

Thwe PM, Pelgrom LR, Cooper R, Beauchamp S, Reisz JA, D'Alessandro A, Everts B, Amiel E
Cell metabolism 2017 Sep 5;26(3):558-567.e5

Placental-Specific Overexpression of sFlt-1 Alters Trophoblast Differentiation and Nutrient Transporter Expression in an IUGR Mouse Model.

Kühnel E, Kleff V, Stojanovska V, Kaiser S, Waldschütz R, Herse F, Plösch T, Winterhager E, Gellhaus A
Journal of cellular biochemistry 2017 Jun;118(6):1316-1329

LMP1-mediated glycolysis induces myeloid-derived suppressor cell expansion in nasopharyngeal carcinoma.

Cai TT, Ye SB, Liu YN, He J, Chen QY, Mai HQ, Zhang CX, Cui J, Zhang XS, Busson P, Zeng YX, Li J
PLoS pathogens 2017 Jul;13(7):e1006503

Increased Rat Placental Fatty Acid, but Decreased Amino Acid and Glucose Transporters Potentially Modify Intrauterine Programming.

Nüsken E, Gellhaus A, Kühnel E, Swoboda I, Wohlfarth M, Vohlen C, Schneider H, Dötsch J, Nüsken KD
Journal of cellular biochemistry 2016 Jul;117(7):1594-603

The drs tumor suppressor regulates glucose metabolism via lactate dehydrogenase-B.

Tambe Y, Hasebe M, Kim CJ, Yamamoto A, Inoue H
Molecular carcinogenesis 2016 Jan;55(1):52-63

Cluster analysis of DCE-MRI data identifies regional tracer-kinetic changes after tumor treatment with high intensity focused ultrasound.

Jacobs I, Hectors SJ, Schabel MC, Grüll H, Strijkers GJ, Nicolay K
NMR in biomedicine 2015 Nov;28(11):1443-54

Enhancement of Neurotrophic Factors in Astrocyte for Neuroprotective Effects in Brain Disorders Using Low-intensity Pulsed Ultrasound Stimulation.

Yang FY, Lu WW, Lin WT, Chang CW, Huang SL
Brain stimulation 2015 May-Jun;8(3):465-73

Bioenergetics profile of CD4(+) T cells in relapsing remitting multiple sclerosis subjects.

De Riccardis L, Rizzello A, Ferramosca A, Urso E, De Robertis F, Danieli A, Giudetti AM, Trianni G, Zara V, Maffia M
Journal of biotechnology 2015 May 20;202:31-9

Metabolic requirements for neutrophil extracellular traps formation.

Rodríguez-Espinosa O, Rojas-Espinosa O, Moreno-Altamirano MM, López-Villegas EO, Sánchez-García FJ
Immunology 2015 Jun;145(2):213-24

An in vitro blood-brain barrier model combining shear stress and endothelial cell/astrocyte co-culture.

Takeshita Y, Obermeier B, Cotleur A, Sano Y, Kanda T, Ransohoff RM
Journal of neuroscience methods 2014 Jul 30;232:165-72

Cited2 is required in trophoblasts for correct placental capillary patterning.

Moreau JL, Artap ST, Shi H, Chapman G, Leone G, Sparrow DB, Dunwoodie SL
Developmental biology 2014 Aug 1;392(1):62-79

Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression.

Luo W, Chang R, Zhong J, Pandey A, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2012 Dec 4;109(49):E3367-76

Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1.

Luo W, Hu H, Chang R, Zhong J, Knabel M, O'Meally R, Cole RN, Pandey A, Semenza GL
Cell 2011 May 27;145(5):732-44

Regulation of TFEB and V-ATPases by mTORC1.

Peña-Llopis S, Vega-Rubin-de-Celis S, Schwartz JC, Wolff NC, Tran TA, Zou L, Xie XJ, Corey DR, Brugarolas J
The EMBO journal 2011 Jul 29;30(16):3242-58

Unique microRNA profile in end-stage heart failure indicates alterations in specific cardiovascular signaling networks.

Naga Prasad SV, Duan ZH, Gupta MK, Surampudi VS, Volinia S, Calin GA, Liu CG, Kotwal A, Moravec CS, Starling RC, Perez DM, Sen S, Wu Q, Plow EF, Croce CM, Karnik S
The Journal of biological chemistry 2009 Oct 2;284(40):27487-99

Priming-dependent phosphorylation and regulation of the tumor suppressor pVHL by glycogen synthase kinase 3.

Hergovich A, Lisztwan J, Thoma CR, Wirbelauer C, Barry RE, Krek W
Molecular and cellular biology 2006 Aug;26(15):5784-96

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Glut1 Antibody [NB110-39113] - Analysis of HeLa lysates using NB110-39113. Image courtesy of Gregg Semenza (PMID: 21620138).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Glut1 Antibody [NB110-39113] - Western blot of GLUT1 on mouse kidney membrane protein (lane A) and rat kidney membrane protein (lane B).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Glut1 Antibody [NB110-39113] - GLUT 1 in A549 cells. Image from verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Glut1 Antibody [NB110-39113] - Glut1 in human brain lysate (55 kDa). Antibody at 1:500. Western blot image submitted by a verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - Immunohistochemical analysis of FFPE tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB detection method and the sections were further counterstained with hematoxylin. This antibody generated a specific strong membrane cytoplasmic staining of Glut1 primarily in the syncytiotrophoblast layers of various villi and in the red blood cells (RBCs). Cytotrophoblasts showed a very weak expression of this protein.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - Analysis of FFPE tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB detection method and the sections were further counterstained with hematoxylin. This antibody generated a specific strong membrane cytoplasmic staining of Glut1 primarily in the syncytiotrophoblast layers of various villi and in the red blood cells (RBCs). Cytotrophoblasts showed a very weak expression of this protein.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: Glut1 Antibody [NB110-39113] - Flow cytometry analysis using the PE conjugate of NB110-39113. Staining of Glut 1 expression on CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. FMO control (red) and isotype control (blue, NBP2-24983) were compared to CD4+ T cells (orange), and this PE conjugated Glut 1 antibody positively stained CD4+ lymphocytes isolated from Mouse. Flow cytometry image submitted by a verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: Glut1 Antibody [NB110-39113] - An intracellular stain was performed on HepG2 with NB110-39113 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: Glut1 Antibody [NB110-39113] - An intracellular stain was performed on HepG cells with Glut1 Antibody NB110-39113F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.