PA5-16793
antibody from Invitrogen Antibodies
Targeting: SLC2A1
CSE, DYT18, DYT9, GLUT, GLUT1, HTLVR
Antibody data
- Antibody Data
- Antigen structure
- References [37]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [10]
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Validation data
Reference
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- Product number
- PA5-16793 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLUT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-16793 targets GLUT-1 in IHC (P), WB, and IF applications and shows reactivity with Human and Rat samples.
- Concentration
- 0.4 mg/mL
Submitted references KEAP1 deficiency drives glucose dependency and sensitizes lung cancer cells and tumors to GLUT inhibition.
Placental Glucose Transporters and Response to Bisphenol A in Pregnancies from of Normal and Overweight Mothers.
NOGOB receptor-mediated RAS signaling pathway is a target for suppressing proliferating hemangioma.
The positive feedback between ACSL4 expression and O-GlcNAcylation contributes to the growth and survival of hepatocellular carcinoma.
Steroids Regulate SLC2A1 and SLC2A3 to Deliver Glucose Into Trophectoderm for Metabolism via Glycolysis.
Cystine transporter regulation of pentose phosphate pathway dependency and disulfide stress exposes a targetable metabolic vulnerability in cancer.
Ibrutinib Resistance Is Reduced by an Inhibitor of Fatty Acid Oxidation in Primary CLL Lymphocytes.
Natural polyphenols as sirtuin 6 modulators.
Galectin-3 induced by hypoxia promotes cell migration in thyroid cancer cells.
Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors.
Inhibition of hypoxia inducible factor 1 and topoisomerase with acriflavine sensitizes perihilar cholangiocarcinomas to photodynamic therapy.
Autophagy Correlates with the Therapeutic Responsiveness of Malignant Pleural Mesothelioma in 3D Models.
Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia.
Multiparametric MRI as an early biomarker of individual therapy effects during concomitant treatment of brain tumours.
Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling.
Gpr124 controls CNS angiogenesis and blood-brain barrier integrity by promoting ligand-specific canonical wnt signaling.
Monitoring hypoxia and vasculature during bevacizumab treatment in a murine colorectal cancer model.
Immunohistochemical expression of GLUT-1 and Ki-67 in chronic plaque psoriasis.
Congenital melanocytic nevus with features of hybrid schwannoma/perineurioma.
Fusion of [(18)F]FDG PET with fluorescence diffuse optical tomography to improve validation of probes and tumor imaging.
Synchrotron microbeam radiation therapy induces hypoxia in intracerebral gliosarcoma but not in the normal brain.
Kaposiform hemangioendothelioma complicated by Kasabach-Merritt phenomenon: ultrastructural observation and immunohistochemistry staining reveal the trapping of blood components.
18F-fluorodeoxyglucose positron emission tomography/computed tomography and the relationship between fluorodeoxyglucose uptake and the expression of hypoxia-inducible factor-1α, glucose transporter-1 and vascular endothelial growth factor in thymic epithelial tumours.
Transforming growth factor-β1 may be a key mediator of the fibrogenic properties of neural cells in leprosy.
Subfield-specific neurovascular remodeling in the entorhino-hippocampal-organotypic slice culture as a response to oxygen-glucose deprivation and excitotoxic cell death.
The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability.
Low-grade fibromyxoid sarcoma with prominent giant rosettes and heterotopic ossification.
Correlation between FDG uptake by PET/CT and the expressions of glucose transporter type 1 and hexokinase II in cervical cancer.
FDG-PET is a good biomarker of both early response and acquired resistance in BRAFV600 mutant melanomas treated with vemurafenib and the MEK inhibitor GDC-0973.
Differences in metabolism between adeno- and squamous cell non-small cell lung carcinomas: spatial distribution and prognostic value of GLUT1 and MCT4.
Targeting Glut1-overexpressing MDA-MB-231 cells with 2-deoxy-D-g1ucose modified SPIOs.
The Warburg effect is genetically determined in inherited pheochromocytomas.
Biological factors, tumor growth kinetics, and survival after metastasectomy for pulmonary melanoma.
GLUT1 expression is increased in hepatocellular carcinoma and promotes tumorigenesis.
Sphingosine kinase 1: a new modulator of hypoxia inducible factor 1alpha during hypoxia in human cancer cells.
Correlation between FDG uptake and glucose transporter type 1 expression in neuroendocrine tumors of the lung.
Human monocyte-derived hemangioma-like endothelial cells: evidence from an in vitro study.
Koppula P, Olszewski K, Zhang Y, Kondiparthi L, Liu X, Lei G, Das M, Fang B, Poyurovsky MV, Gan B
iScience 2021 Jun 25;24(6):102649
iScience 2021 Jun 25;24(6):102649
Placental Glucose Transporters and Response to Bisphenol A in Pregnancies from of Normal and Overweight Mothers.
Ermini L, Nuzzo AM, Ietta F, Romagnoli R, Moretti L, Masturzo B, Paulesu L, Rolfo A
International journal of molecular sciences 2021 Jun 21;22(12)
International journal of molecular sciences 2021 Jun 21;22(12)
NOGOB receptor-mediated RAS signaling pathway is a target for suppressing proliferating hemangioma.
Hu W, Liu Z, Salato V, North PE, Bischoff J, Kumar SN, Fang Z, Rajan S, Hussain MM, Miao QR
JCI insight 2021 Feb 8;6(3)
JCI insight 2021 Feb 8;6(3)
The positive feedback between ACSL4 expression and O-GlcNAcylation contributes to the growth and survival of hepatocellular carcinoma.
Wang J, Wang Z, Yuan J, Wang J, Shen X
Aging 2020 May 1;12(9):7786-7800
Aging 2020 May 1;12(9):7786-7800
Steroids Regulate SLC2A1 and SLC2A3 to Deliver Glucose Into Trophectoderm for Metabolism via Glycolysis.
Kramer AC, Steinhauser CB, Gao H, Seo H, McLendon BA, Burghardt RC, Wu G, Bazer FW, Johnson GA
Endocrinology 2020 Aug 1;161(8)
Endocrinology 2020 Aug 1;161(8)
Cystine transporter regulation of pentose phosphate pathway dependency and disulfide stress exposes a targetable metabolic vulnerability in cancer.
Liu X, Olszewski K, Zhang Y, Lim EW, Shi J, Zhang X, Zhang J, Lee H, Koppula P, Lei G, Zhuang L, You MJ, Fang B, Li W, Metallo CM, Poyurovsky MV, Gan B
Nature cell biology 2020 Apr;22(4):476-486
Nature cell biology 2020 Apr;22(4):476-486
Ibrutinib Resistance Is Reduced by an Inhibitor of Fatty Acid Oxidation in Primary CLL Lymphocytes.
Galicia-Vázquez G, Aloyz R
Frontiers in oncology 2018;8:411
Frontiers in oncology 2018;8:411
Natural polyphenols as sirtuin 6 modulators.
Rahnasto-Rilla M, Tyni J, Huovinen M, Jarho E, Kulikowicz T, Ravichandran S, A Bohr V, Ferrucci L, Lahtela-Kakkonen M, Moaddel R
Scientific reports 2018 Mar 7;8(1):4163
Scientific reports 2018 Mar 7;8(1):4163
Galectin-3 induced by hypoxia promotes cell migration in thyroid cancer cells.
Zheng J, Lu W, Wang C, Xing Y, Chen X, Ai Z
Oncotarget 2017 Nov 24;8(60):101475-101488
Oncotarget 2017 Nov 24;8(60):101475-101488
Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors.
Cogger KF, Sinha A, Sarangi F, McGaugh EC, Saunders D, Dorrell C, Mejia-Guerrero S, Aghazadeh Y, Rourke JL, Screaton RA, Grompe M, Streeter PR, Powers AC, Brissova M, Kislinger T, Nostro MC
Nature communications 2017 Aug 24;8(1):331
Nature communications 2017 Aug 24;8(1):331
Inhibition of hypoxia inducible factor 1 and topoisomerase with acriflavine sensitizes perihilar cholangiocarcinomas to photodynamic therapy.
Weijer R, Broekgaarden M, Krekorian M, Alles LK, van Wijk AC, Mackaaij C, Verheij J, van der Wal AC, van Gulik TM, Storm G, Heger M
Oncotarget 2016 Jan 19;7(3):3341-56
Oncotarget 2016 Jan 19;7(3):3341-56
Autophagy Correlates with the Therapeutic Responsiveness of Malignant Pleural Mesothelioma in 3D Models.
Barbone D, Follo C, Echeverry N, Gerbaudo VH, Klabatsa A, Bueno R, Felley-Bosco E, Broaddus VC
PloS one 2015;10(8):e0134825
PloS one 2015;10(8):e0134825
Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia.
Sun Y, George J, Rocha S
PloS one 2015;10(4):e0123649
PloS one 2015;10(4):e0123649
Multiparametric MRI as an early biomarker of individual therapy effects during concomitant treatment of brain tumours.
Lemasson B, Bouchet A, Maisin C, Christen T, Le Duc G, Rémy C, Barbier EL, Serduc R
NMR in biomedicine 2015 Sep;28(9):1163-73
NMR in biomedicine 2015 Sep;28(9):1163-73
Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling.
Pan PY, Marrs J, Ryan TA
The Journal of biological chemistry 2015 Sep 11;290(37):22593-601
The Journal of biological chemistry 2015 Sep 11;290(37):22593-601
Gpr124 controls CNS angiogenesis and blood-brain barrier integrity by promoting ligand-specific canonical wnt signaling.
Zhou Y, Nathans J
Developmental cell 2014 Oct 27;31(2):248-56
Developmental cell 2014 Oct 27;31(2):248-56
Monitoring hypoxia and vasculature during bevacizumab treatment in a murine colorectal cancer model.
Heijmen L, Ter Voert EG, Punt CJ, Heerschap A, Oyen WJ, Bussink J, Sweep CG, Laverman P, Span PN, de Geus-Oei LF, Boerman OC, van Laarhoven HW
Contrast media & molecular imaging 2014 May-Jun;9(3):237-45
Contrast media & molecular imaging 2014 May-Jun;9(3):237-45
Immunohistochemical expression of GLUT-1 and Ki-67 in chronic plaque psoriasis.
Abdou AG, Maraee AH, Eltahmoudy M, El-Aziz RA
The American Journal of dermatopathology 2013 Oct;35(7):731-7
The American Journal of dermatopathology 2013 Oct;35(7):731-7
Congenital melanocytic nevus with features of hybrid schwannoma/perineurioma.
Wang L, Wang G, Gao T
Journal of cutaneous pathology 2013 May;40(5):497-502
Journal of cutaneous pathology 2013 May;40(5):497-502
Fusion of [(18)F]FDG PET with fluorescence diffuse optical tomography to improve validation of probes and tumor imaging.
Garofalakis A, Dubois A, Thézé B, Czarny B, Tavitian B, Ducongé F
Molecular imaging and biology 2013 Jun;15(3):316-25
Molecular imaging and biology 2013 Jun;15(3):316-25
Synchrotron microbeam radiation therapy induces hypoxia in intracerebral gliosarcoma but not in the normal brain.
Bouchet A, Lemasson B, Christen T, Potez M, Rome C, Coquery N, Le Clec'h C, Moisan A, Bräuer-Krisch E, Leduc G, Rémy C, Laissue JA, Barbier EL, Brun E, Serduc R
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2013 Jul;108(1):143-8
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2013 Jul;108(1):143-8
Kaposiform hemangioendothelioma complicated by Kasabach-Merritt phenomenon: ultrastructural observation and immunohistochemistry staining reveal the trapping of blood components.
Yuan SM, Hong ZJ, Chen HN, Shen WM, Zhou XJ
Ultrastructural pathology 2013 Dec;37(6):452-5
Ultrastructural pathology 2013 Dec;37(6):452-5
18F-fluorodeoxyglucose positron emission tomography/computed tomography and the relationship between fluorodeoxyglucose uptake and the expression of hypoxia-inducible factor-1α, glucose transporter-1 and vascular endothelial growth factor in thymic epithelial tumours.
Toba H, Kondo K, Sadohara Y, Otsuka H, Morimoto M, Kajiura K, Nakagawa Y, Yoshida M, Kawakami Y, Takizawa H, Kenzaki K, Sakiyama S, Bando Y, Tangoku A
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 2013 Aug;44(2):e105-12
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 2013 Aug;44(2):e105-12
Transforming growth factor-β1 may be a key mediator of the fibrogenic properties of neural cells in leprosy.
Petito RB, Amadeu TP, Pascarelli BM, Jardim MR, Vital RT, Antunes SL, Sarno EN
Journal of neuropathology and experimental neurology 2013 Apr;72(4):351-66
Journal of neuropathology and experimental neurology 2013 Apr;72(4):351-66
Subfield-specific neurovascular remodeling in the entorhino-hippocampal-organotypic slice culture as a response to oxygen-glucose deprivation and excitotoxic cell death.
Chip S, Nitsch C, Wellmann S, Kapfhammer JP
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2013 Apr;33(4):508-18
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2013 Apr;33(4):508-18
The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability.
Bett JS, Ibrahim AF, Garg AK, Kelly V, Pedrioli P, Rocha S, Hay RT
The Biochemical journal 2013 Apr 15;451(2):185-94
The Biochemical journal 2013 Apr 15;451(2):185-94
Low-grade fibromyxoid sarcoma with prominent giant rosettes and heterotopic ossification.
Hisaoka M, Matsuyama A, Aoki T, Sakamoto A, Yokoyama K
Pathology, research and practice 2012 Sep 15;208(9):557-60
Pathology, research and practice 2012 Sep 15;208(9):557-60
Correlation between FDG uptake by PET/CT and the expressions of glucose transporter type 1 and hexokinase II in cervical cancer.
Tong SY, Lee JM, Ki KD, Choi YJ, Seol HJ, Lee SK, Huh CY, Kim GY, Lim SJ
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society 2012 May;22(4):654-8
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society 2012 May;22(4):654-8
FDG-PET is a good biomarker of both early response and acquired resistance in BRAFV600 mutant melanomas treated with vemurafenib and the MEK inhibitor GDC-0973.
Baudy AR, Dogan T, Flores-Mercado JE, Hoeflich KP, Su F, van Bruggen N, Williams SP
EJNMMI research 2012 May 31;2(1):22
EJNMMI research 2012 May 31;2(1):22
Differences in metabolism between adeno- and squamous cell non-small cell lung carcinomas: spatial distribution and prognostic value of GLUT1 and MCT4.
Meijer TW, Schuurbiers OC, Kaanders JH, Looijen-Salamon MG, de Geus-Oei LF, Verhagen AF, Lok J, van der Heijden HF, Rademakers SE, Span PN, Bussink J
Lung cancer (Amsterdam, Netherlands) 2012 Jun;76(3):316-23
Lung cancer (Amsterdam, Netherlands) 2012 Jun;76(3):316-23
Targeting Glut1-overexpressing MDA-MB-231 cells with 2-deoxy-D-g1ucose modified SPIOs.
Shan XH, Hu H, Xiong F, Gu N, Geng XD, Zhu W, Lin J, Wang YF
European journal of radiology 2012 Jan;81(1):95-9
European journal of radiology 2012 Jan;81(1):95-9
The Warburg effect is genetically determined in inherited pheochromocytomas.
Favier J, Brière JJ, Burnichon N, Rivière J, Vescovo L, Benit P, Giscos-Douriez I, De Reyniès A, Bertherat J, Badoual C, Tissier F, Amar L, Libé R, Plouin PF, Jeunemaitre X, Rustin P, Gimenez-Roqueplo AP
PloS one 2009 Sep 18;4(9):e7094
PloS one 2009 Sep 18;4(9):e7094
Biological factors, tumor growth kinetics, and survival after metastasectomy for pulmonary melanoma.
Lee JH, Gulec SA, Kyshtoobayeva A, Sim MS, Morton DL
Annals of surgical oncology 2009 Oct;16(10):2834-9
Annals of surgical oncology 2009 Oct;16(10):2834-9
GLUT1 expression is increased in hepatocellular carcinoma and promotes tumorigenesis.
Amann T, Maegdefrau U, Hartmann A, Agaimy A, Marienhagen J, Weiss TS, Stoeltzing O, Warnecke C, Schölmerich J, Oefner PJ, Kreutz M, Bosserhoff AK, Hellerbrand C
The American journal of pathology 2009 Apr;174(4):1544-52
The American journal of pathology 2009 Apr;174(4):1544-52
Sphingosine kinase 1: a new modulator of hypoxia inducible factor 1alpha during hypoxia in human cancer cells.
Ader I, Brizuela L, Bouquerel P, Malavaud B, Cuvillier O
Cancer research 2008 Oct 15;68(20):8635-42
Cancer research 2008 Oct 15;68(20):8635-42
Correlation between FDG uptake and glucose transporter type 1 expression in neuroendocrine tumors of the lung.
Song YS, Lee WW, Chung JH, Park SY, Kim YK, Kim SE
Lung cancer (Amsterdam, Netherlands) 2008 Jul;61(1):54-60
Lung cancer (Amsterdam, Netherlands) 2008 Jul;61(1):54-60
Human monocyte-derived hemangioma-like endothelial cells: evidence from an in vitro study.
Jia J, Huang X, Zhang WF, Zhao YF
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology 2008 Jul-Aug;17(4):212-8
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology 2008 Jul-Aug;17(4):212-8
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Supportive validation
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- Western blot of GLUT-1 using GLUT-1 Polyclonal Antibody (Product # PA5-16793) on Rat Brain Lysate.
Supportive validation
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- Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with Glut-1 antibody using peroxidase-conjugate and AEC chromogen. Note membrane staining of tumor cells.
Supportive validation
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- Fig. 1 Imaging using IRDye800CW 2-DG and [ 18 F]FDG. ( a ) Sagittal view of the [ 18 F]FDG-PET signal of a xenografted mouse showing high activity at the site of the xenografted tumor (tumor volume of 775 mm 3 ). The arrow pinpoints the position of the tumor as identified by [ 18 F]FDG. ( b ) The fDOT IRDye800CW 2-DG signal at the corresponding sagittal plane. The area, in which the fDOT reconstruction is performed, is depicted by a dotted white frame . ( c ) Fused PET/fDOT sagittal image originating from the images of ( a ) and ( b ). ( d ) Sagittal view of the volumes occupied by the fDOT ( blue ) and PET ( red ) signal rendered to the 3D surface of the mouse ( gray ). ( e , f , and g ) The equivalents of ( a ), ( b ), and ( c ) are presented, but in the axial view; ( h ) Axial view of fused fDOT/CT image at the level of the tumor. ( i ) Immunohistochemical labeling for GLUT-1 expression ( in red ) in the excised tumor. Nuclei ( in blue ) are counterstained with DAPI. ( j ) Fluorescence image shows the biodistribution of IRDye800CW 2-DG signal in the same section than ( i ). ( k ) Image of the fusion of ( i ) and ( j ) shows the co-localization of the GLUT-1 and the IRDye800CW 2-DG. ( l ) White light image of the whole animal axial section.
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- Figure 4 ACSL4 upregulation enhanced the stability of GLUT1 protein and reduced its ubiquitination. ( A ) After 12 hours of cell transfection with si-ACSL4 or OE-ACSL4, Huh-7 cells were treated with CHX (100 mug/mL) for 0, 1, 2, 4, 8 or 24 hours, and the western blotting assay was performed to detect GLUT1 expression. ( B ) An IP assay was used to detect the interaction between Ub and GLUT1 proteins after Huh-7 cells were transfected with si-ACSL4 or OE-ACSL4. ( C ) After 12 hours of cell transfection with si-ACSL4 or OE-ACSL4, SK-HEP-1 cells were treated with CHX (100 mug/mL) for 0, 1, 2, 4, 8 or 24 hours, and the western blotting assay was performed to detect GLUT1 expression. ( D ) IP assay was used to detect the interaction between Ub and GLUT1 protein in SK-HEP-1 cells. ( * P
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- Figure 5 Evaluation of the effects of the ACSL4/GLUT1 axis on cell proliferation, apoptosis and tumorigenesis in Huh-7 and SK-HEP-1 cells. ( A , B ) The mRNA and protein expression levels of GLUT1 were determined by RT-PCR and western blotting assays after cells were transfected with sh-GLUT1 or sh-NC, respectively ( * P
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- Figure 6 Evaluation of the levels of GLUT1 and O-GlcNAc in HCC and their clinical significance. ( A ) Immunohistochemistry technology was used to assess the protein levels of ACSL4, O-GlcNAc and GLUT1 in HCC tissues and adjacent normal tissues (Scale bar = 100 mum). ( B - D ) Pearson correlation analysis of the correlations between the levels of ACSL4, O-GlcNAc and GLUT1 in HCC tissues. ( E , F ) Kaplan-Meier analysis of the relationship between GLUT1/O-GlcNAc levels and the overall survival of patients with HCC.
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- Figure 1 Glut 1 and 4 expression ( A , C ) and protein levels ( B , D ) in placenta from normal ( CTRL ) and overweight ( OW ) women. ( A ) qPCR analysis of GLUT1 mRNA in normal ( n = 32) and overweight ( n = 26) placental tissue. ( B ) Representative WB (high panel) and corresponding densitometry (low panel) of GLUT1 in placenta from CTRL ( n = 12) and OW ( n = 18) women. ( C ) qPCR analysis of GLUT4 mRNA in normal ( n = 26) and overweight ( n = 25) placental tissue. ( D ) Representative WB (high panel) and corresponding densitometry (low panel) of GLUT4 in placenta from CTRL ( n = 12) and OW ( n = 18) women. Data are presented as mean +-ES.
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- Figure 2 GLUT 1 and 4 expression ( A , C ) and protein levels ( B , D ) in placenta explants from normal ( CTRL ) and overweight ( OW ) women. ( A ) Fold Change of GLUT1 mRNA in normal ( n = 3) and overweight ( n = 5) placental explants. ( B ) Representative WB (left panel) and corresponding densitometry (right panel) of GLUT1 in placenta explants from CTRL ( n = 6) and OW ( n = 5) women. ( C ) Fold Change of GLUT4 mRNA in normal ( n = 3) and overweight ( n = 5) placental explants. ( D ) Representative WB (left panel) and corresponding densitometry (right panel) of GLUT4 in placenta explants from CTRL ( n = 6) and OW ( n = 5) women. Data are presented as mean +-ES. Significance was determined using an unpaired two-sided t -test. * p < 0.05.
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- Figure 3 GLUT 1 and 4 expressions ( A , C ) and protein levels ( B , D ) in placenta explants treated with BPA from control pregnancy. ( A ) Fold Change of GLUT1 mRNA in placental explants from NW women ( n = 3) treated with BPA 1 nM and BPA 1 muM compared to the vehicle as control. ( B ) Representative WB (left panel) and corresponding densitometry (right panel) of GLUT1 in placental explants from NW women ( n = 4) treated with BPA 1 nM and BPA 1 muM compared to the vehicle as control. ( C ) Fold Change of GLUT4 mRNA in placental explants from NW women ( n = 3) treated with BPA 1 nM and BPA 1 muM compared to the vehicle as control. ( D ) Representative WB (left panel) and corresponding densitometry (right panel) of GLUT4 in placental explants from NW women ( n = 4) treated with BPA 1 nM and BPA 1 muM compared to the vehicle as control. Data are presented as mean +-ES. Significance was determined using a one-way ANOVA and Bonferroni's test for post hoc comparisons * p < 0.05. Ct: control.
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- Figure 4 GLUT 1 and 4 expressions ( A , C ) and protein levels ( B , D ) in placenta explants treated with BPA from overweight women. ( A ) Fold Change of GLUT1 mRNA in placental explants from OW women ( n = 5) treated with BPA 1 nM and BPA 1 muM compared to the vehicle as control. ( B ) Representative WB (left panel) and corresponding densitometry (right panel) of GLUT1 in placental explants from OW women ( n = 6) treated with BPA 1 nM and BPA 1 muM compared to the vehicle as control. ( C ) Fold Change of GLUT4 mRNA in placental explants from OW women ( n = 5) treated with BPA 1 nM and BPA 1 muM compared to the vehicle as control. ( D ) Representative WB (left panel) and corresponding densitometry (right panel) of GLUT4 in placental explants from OW women ( n = 6) treated with BPA 1 nM and BPA 1 muM compared to the vehicle as control. Data are presented as mean +-ES. Significance was determined using a one-way ANOVA and Bonferroni's test for post hoc comparisons * p < 0.05. Ct: control.
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- Fig. 7 Functional and phenotypical characterization of presort- and GP2 + -derived aggregates. a Insulin secretion (ng insulin/mug DNA) from three different batches of aggregates generated from H1-derived presort and GP2 + cells at day 25, 24, and 22 of differentiation. N = 6 of 20-25 representative aggregates treated with low (2.8 mM) and high (16.7 mM) glucose and KCl (45 mM) for 45 min, error bars indicate s.e.m. b INSULIN (INS), NKX2-2, GLUT1 and MAFA immunostaining of unsorted (PRESORT) and GP2 + H1 cells at day 23 of differentiation. Scale bar represents 100 mum