Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB1418 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Glut1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Glut1. Stains human Glut1-transfected NS0 cells, but not NS0 control transfectants. Although Human Glut1 Antibody detects Glut1 on the surface of T cells (1, 2), it does not detect it on erythrocytes (5). The reason for this discrepancy is not understood, but may be related to conformational or post-translational modification differences.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AAA52571- Isotype
- IgG
- Antibody clone number
- 202915
- Vial size
- 200 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Detection and Characterization of CD8+ Autoreactive Memory Stem T Cells in Patients With Type 1 Diabetes.
Rhinovirus induces an anabolic reprogramming in host cell metabolism essential for viral replication.
Manipulating Glucose Metabolism during Different Stages of Viral Pathogenesis Can Have either Detrimental or Beneficial Effects.
Emerging Role and Characterization of Immunometabolism: Relevance to HIV Pathogenesis, Serious Non-AIDS Events, and a Cure.
Foxp3-mediated inhibition of Akt inhibits Glut1 (glucose transporter 1) expression in human T regulatory cells.
Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection.
Role of FDG-PET as a biological marker for predicting the hypoxic status of tongue cancer.
Entry of human T-cell leukemia virus type 1 is augmented by heparin sulfate proteoglycans bearing short heparin-like structures.
The chemokine CCL5 regulates glucose uptake and AMP kinase signaling in activated T cells to facilitate chemotaxis.
DC-SIGN mediates cell-free infection and transmission of human T-cell lymphotropic virus type 1 by dendritic cells.
Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates.
Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates.
Vignali D, Cantarelli E, Bordignon C, Canu A, Citro A, Annoni A, Piemonti L, Monti P
Diabetes 2018 May;67(5):936-945
Diabetes 2018 May;67(5):936-945
Rhinovirus induces an anabolic reprogramming in host cell metabolism essential for viral replication.
Gualdoni GA, Mayer KA, Kapsch AM, Kreuzberg K, Puck A, Kienzl P, Oberndorfer F, Frühwirth K, Winkler S, Blaas D, Zlabinger GJ, Stöckl J
Proceedings of the National Academy of Sciences of the United States of America 2018 Jul 24;115(30):E7158-E7165
Proceedings of the National Academy of Sciences of the United States of America 2018 Jul 24;115(30):E7158-E7165
Manipulating Glucose Metabolism during Different Stages of Viral Pathogenesis Can Have either Detrimental or Beneficial Effects.
Varanasi SK, Donohoe D, Jaggi U, Rouse BT
Journal of immunology (Baltimore, Md. : 1950) 2017 Sep 1;199(5):1748-1761
Journal of immunology (Baltimore, Md. : 1950) 2017 Sep 1;199(5):1748-1761
Emerging Role and Characterization of Immunometabolism: Relevance to HIV Pathogenesis, Serious Non-AIDS Events, and a Cure.
Palmer CS, Henstridge DC, Yu D, Singh A, Balderson B, Duette G, Cherry CL, Anzinger JJ, Ostrowski M, Crowe SM
Journal of immunology (Baltimore, Md. : 1950) 2016 Jun 1;196(11):4437-44
Journal of immunology (Baltimore, Md. : 1950) 2016 Jun 1;196(11):4437-44
Foxp3-mediated inhibition of Akt inhibits Glut1 (glucose transporter 1) expression in human T regulatory cells.
Basu S, Hubbard B, Shevach EM
Journal of leukocyte biology 2015 Feb;97(2):279-83
Journal of leukocyte biology 2015 Feb;97(2):279-83
Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection.
Palmer CS, Ostrowski M, Gouillou M, Tsai L, Yu D, Zhou J, Henstridge DC, Maisa A, Hearps AC, Lewin SR, Landay A, Jaworowski A, McCune JM, Crowe SM
AIDS (London, England) 2014 Jan 28;28(3):297-309
AIDS (London, England) 2014 Jan 28;28(3):297-309
Role of FDG-PET as a biological marker for predicting the hypoxic status of tongue cancer.
Han MW, Lee HJ, Cho KJ, Kim JS, Roh JL, Choi SH, Nam SY, Kim SY
Head & neck 2012 Oct;34(10):1395-402
Head & neck 2012 Oct;34(10):1395-402
Entry of human T-cell leukemia virus type 1 is augmented by heparin sulfate proteoglycans bearing short heparin-like structures.
Tanaka A, Jinno-Oue A, Shimizu N, Hoque A, Mori T, Islam S, Nakatani Y, Shinagawa M, Hoshino H
Journal of virology 2012 Mar;86(6):2959-69
Journal of virology 2012 Mar;86(6):2959-69
The chemokine CCL5 regulates glucose uptake and AMP kinase signaling in activated T cells to facilitate chemotaxis.
Chan O, Burke JD, Gao DF, Fish EN
The Journal of biological chemistry 2012 Aug 24;287(35):29406-16
The Journal of biological chemistry 2012 Aug 24;287(35):29406-16
DC-SIGN mediates cell-free infection and transmission of human T-cell lymphotropic virus type 1 by dendritic cells.
Jain P, Manuel SL, Khan ZK, Ahuja J, Quann K, Wigdahl B
Journal of virology 2009 Nov;83(21):10908-21
Journal of virology 2009 Nov;83(21):10908-21
Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates.
Luty WH, Rodeberg D, Parness J, Vyas YM
Journal of immunology (Baltimore, Md. : 1950) 2007 Jul 15;179(2):819-29
Journal of immunology (Baltimore, Md. : 1950) 2007 Jul 15;179(2):819-29
Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates.
Luty WH, Rodeberg D, Parness J, Vyas YM
Journal of immunology (Baltimore, Md. : 1950) 2007 Jul 15;179(2):819-29
Journal of immunology (Baltimore, Md. : 1950) 2007 Jul 15;179(2):819-29
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Glut1 in HepG2 Human Hepatocellular Carcinoma Cell Line. Glut1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Glut1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line either (A) untreated or (B) cultured in nutrient-depleted media was stained with Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
