- GTX104557 - GeneTex GPC1 antibody

Submitted references Abnormal ER quality control of neural GPI-anchored proteins via dysfunction in ER export processing in the frontal cortex of elderly subjects with schizophrenia.

Kim P, Scott MR, Meador-Woodruff JH
Translational psychiatry 2019 Jan 16;9(1):6

Sphingosine-1-phosphate improves endothelialization with reduction of thrombosis in recellularized human umbilical vein graft by inhibiting syndecan-1 shedding in vitro.

Hsia K, Yang MJ, Chen WM, Yao CL, Lin CH, Loong CC, Huang YL, Lin YT, Lander AD, Lee H, Lu JH
Acta biomaterialia 2017 Mar 15;51:341-350

Elevated glypican-1 expression is associated with an unfavorable prognosis in pancreatic ductal adenocarcinoma.

Lu H, Niu F, Liu F, Gao J, Sun Y, Zhao X
Cancer medicine 2017 Jun;6(6):1181-1191

α3 Chains of type V collagen regulate breast tumour growth via glypican-1.

Huang G, Ge G, Izzi V, Greenspan DS
Nature communications 2017 Jan 19;8:14351

Differential expression of glypican-1 in ameloblastoma variants.

Bologna-Molina R, Mosqueda-Taylor A, Molina-Frechero N
Applied immunohistochemistry & molecular morphology : AIMM 2015 Feb;23(2):153-60

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) MCF-7 whole cell extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Prod no: Proprietary.
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
Antibody Lot Number: 41983

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Supportive validation

Submitted by: GeneTex (provider)
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Experimental details: Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) B: Molt-4 (GTX27912) 7.5% SDS PAGE GTX104557 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) A431 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) MDA-MB-231 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: U87-MG whole cell and membrane extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: U87-MG whole cell extract and conditioned medium (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Mouse tissue extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) A431 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500 and competitor's antibody (sc-365000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) MCF-7 whole cell extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 antibody (GTX104557) at concentration ranged from 7.5 to 240 ng/mL.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Glypican 1 antibody [N3C3] detects Glypican 1 protein at endoplasmic reticulum by immunofluorescent analysis.Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min.Green: Glypican 1 protein stained by Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:400.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Glypican 1 antibody [N3C3] detects Glypican 1 protein at cytoplasm by immunofluorescent analysis.Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min.Green: Glypican 1 protein stained by Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of Glypican 1 protein from MCF-7 whole cell extracts using 5 £gg of Glypican 1 antibody [N3C3] (GTX104557).Western blot analysis was performed using Glypican 1 antibody [N3C3] (GTX104557).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical microscopy analysis of paraffin-embedded human normal pancreas (left) or pancreatic adenocarcinoma (grade II) (right) tissues using Glypican-1 antibody [N3C3] (GTX104557) at a 1:250 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Glypican 1 antibody [N3C3] (GTX104557) detects Glypican 1 protein by flow cytometry analysis. Sample: A431 cell. Black: Unlabelled sample was used as a control. Red: Glypican 1 antibody [N3C3] (GTX104557) dilution: 1:100. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

GTX104557

Antibody data