Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- AF4519 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Glypican 1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Glypican 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse Glypican 1 is observed and less than 1% cross-reactivity with recombinant human (rh) Glypican 5 and rhGlypican 6 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Receptor tyrosine kinase inhibitor Sunitinib and integrin antagonist peptide HM-3 show similar lipid raft dependent biphasic regulation of tumor angiogenesis and metastasis.
Overexpression of glypican-1 implicates poor prognosis and their chemoresistance in oesophageal squamous cell carcinoma.
Hu J, Wang W, Liu C, Li M, Nice E, Xu H
Journal of experimental & clinical cancer research : CR 2019 Aug 28;38(1):381
Journal of experimental & clinical cancer research : CR 2019 Aug 28;38(1):381
Overexpression of glypican-1 implicates poor prognosis and their chemoresistance in oesophageal squamous cell carcinoma.
Hara H, Takahashi T, Serada S, Fujimoto M, Ohkawara T, Nakatsuka R, Harada E, Nishigaki T, Takahashi Y, Nojima S, Miyazaki Y, Makino T, Kurokawa Y, Yamasaki M, Miyata H, Nakajima K, Takiguchi S, Morii E, Mori M, Doki Y, Naka T
British journal of cancer 2016 Jun 28;115(1):66-75
British journal of cancer 2016 Jun 28;115(1):66-75
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Glypican 1 in MDA-MB-231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Goat Anti-Human Glypican 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4519, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).