Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AF4519 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Glypican 1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Glypican 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse Glypican 1 is observed and less than 1% cross-reactivity with recombinant human (rh) Glypican 5 and rhGlypican 6 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P35052
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Overexpression of glypican-1 implicates poor prognosis and their chemoresistance in oesophageal squamous cell carcinoma.
Hara H, Takahashi T, Serada S, Fujimoto M, Ohkawara T, Nakatsuka R, Harada E, Nishigaki T, Takahashi Y, Nojima S, Miyazaki Y, Makino T, Kurokawa Y, Yamasaki M, Miyata H, Nakajima K, Takiguchi S, Morii E, Mori M, Doki Y, Naka T
British journal of cancer 2016 Jun 28;115(1):66-75
British journal of cancer 2016 Jun 28;115(1):66-75
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Glypican 1 in MDA-MB-231 Human Cell Line. Glypican 1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-Human Glypican 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4519) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Glypican 1 in MDA-MB-231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Goat Anti-Human Glypican 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4519, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).