Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BAF4519 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Glypican 1 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Glypican 1 in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse Glypican 1 is observed and less than 1% cross-reactivity with recombinant human (rh)Glypican 5 and rhGlypican 6 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P35052
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Autoregulation of glypican-1 by intronic microRNA-149 fine tunes the angiogenic response to FGF2 in human endothelial cells.
Hemodynamic shear stress characteristic of atherosclerosis-resistant regions promotes glycocalyx formation in cultured endothelial cells.
Chamorro-Jorganes A, Araldi E, Rotllan N, Cirera-Salinas D, Suárez Y
Journal of cell science 2014 Mar 15;127(Pt 6):1169-78
Journal of cell science 2014 Mar 15;127(Pt 6):1169-78
Hemodynamic shear stress characteristic of atherosclerosis-resistant regions promotes glycocalyx formation in cultured endothelial cells.
Koo A, Dewey CF Jr, García-Cardeña G
American journal of physiology. Cell physiology 2013 Jan 15;304(2):C137-46
American journal of physiology. Cell physiology 2013 Jan 15;304(2):C137-46
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Glypican 1 in MDA-MB-231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Goat Anti-Human Glypican 1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4519, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).