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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- NBP2-59371 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal RNPEPL1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Store at -20C.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: RNPEPL1 Antibody (4A9) [NBP2-59371] - Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 (SMC-263). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody (SMC-263) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.
