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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [6]
- Flow cytometry [1]
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- Product number
- 17658-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LGR6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MGRPRLTLV CQVSIIISAR DLSMNNLTEL QPGLFHHLRF LEELRLSGNH LSHIPGQAFS GLYSLKILML QNNQLGGIPA EALWELPSLQ SLRLDANLIS LVPERSFEGL SSLRHLWLDD NALTEIPVRA LNNLPALQAM TLALNRISHI PDYAFQNLTS LVVLHLHNNR IQHLGTHSFE GLHNLETLDL NYNKLQEFPV AIRTLGRLQE LGFHNNNIKA IPEKAFMGNP LLQTIHFYDN PIQFVGRSAF QYLPKLHTLS LNGAMDIQEF PDLKGTTSLE ILTLTRAGIR LLPSGMCQQL PRLRVLELSH NQIEELPSLH RCQKLEEIGL QHNRIWEIGA DTFSQLSSLQ AL (1-351 aa encoded by BC047905)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 17658-1-AP ( LGR6 Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 17658-1-AP ( LGR6 Antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug LGR6 antibody (17658-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
