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- Product number
- MA5-26185 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MYBPH Monoclonal Antibody (OTI3G1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI3G1
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Myogenic regulatory factors are key players in determining muscle mass and meat quality in Jeju native and Berkshire pigs.
Kim K, Kim D, Min Y, Jeong D, Son YO, Do K
Veterinary medicine and science 2021 May;7(3):735-745
Veterinary medicine and science 2021 May;7(3):735-745
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MYBPH in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with MYBPH (Product # MA5-26185) monoclonal antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MYBPH in COS7 cells. Cells were transfected with a plasmid overexpressing MYBPH and probed with a MYBPH monoclonal antibody (Product # MA5-26185).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded carcinoma of human lung tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a MYBPH monoclonal antibody (Product # MA5-26185).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human colon tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a MYBPH monoclonal antibody (Product # MA5-26185).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a MYBPH monoclonal antibody (Product # MA5-26185).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 2 Protein levels of myogenic regulatory factors (MRFs). The protein levels of MyoD, Pax7, Myf5, Myf6 and MyBPH were analysed by western blotting using the longissimus dorsi muscles of 1-day-old piglets and adult pigs of Berkshire and JNPs (a). Protein levels were normalized to beta-actin, and relative intensity of MyoD (b), Pax7 (c), Myf5 (d), Myf6 (e) and MyBPH (f) are represented. Values are presented as mean +- standard error of mean (s.e.m.) (* p < .05, ** p < .01 and *** p < .001; two-tailed t -test). JNP: Jeju native pig
