Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- GTX17309 - Provider product page
- Provider
- GeneTex
- Product name
- CHI3L1 antibody [AT2C10]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Cell lysates of HepG2 (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CHI3L1 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
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- GeneTex (provider)
- Main image
- Experimental details
- GTX17309 CHI3L1 (Clone AT2C10) WB Result Lane 1: Recombinant CHI3L1 (50ng) Lane 2: SW480 Lysate (40ug) Lane 3: HepG2 Lysate (40ug) 1. Blocking: 5% Skim milk in TBST, 1hr 2. 1st Ab: 1:500, 2hr 3. 2nd Ab: 1:10000, 1hr 4. ECL