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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-47841 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRELP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human liver tissue and human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-human PRELP Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47841) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for PRELP at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRELP in human liver tissue and human pancreas tissue. Samples were incubated in PRELP polyclonal antibody (Product # PA5-47841) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for PRELP at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of PRELP in immersion fixed human mesenchymal stem cells differentiated into chondrocytes. Samples were incubated in PRELP polyclonal antibody (Product # PA5-47841) using a dilution of 10 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of PRELP by (Product # PA5-47841) showing the decrease in signal with increasing concentrations of PRELP Antibody. Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of PRELP in RAW 264.7 mouse monocyte/macrophage cell line. Samples were incubated in PRELP polyclonal antibody (Product # PA5-47841). Recombinant Human PRELP inhibits Recombinant Mouse TRANCE induced osteoclast-like cell formation in the RAW 264.7 mouse monocyte/macrophage cell line in a dose-dependent manner (orange line), as measured by the TRAP (tartrate‚resistant acid phosphatase) solution assay. Inhibition of Recombinant Mouse TRANCE (5 ng/mL) activity elicited by Recombinant Human PRELP (5 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human PRELP Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.2-1.2 µg/mL in the presence of Recombinant Mouse M‚CSF (20 ng/mL).
