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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF6447 - Provider product page
- Provider
- R&D Systems
- Product name
- Human PRELP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human PRELP in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P51888- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human PRELP by Western Blot. Western blot shows lysates of human liver tissue and human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PRELP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6447) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PRELP at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- PRELP in Human Mesenchymal Stem Cells. PRELP was detected in immersion fixed human mesenchymal stem cells differentiated into chondrocytes using Sheep Anti-Human PRELP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6447) at 10 µg/mL overnight at 4 °C. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- PRELP Inhibition of TRANCE-dependent Osteoclast-Like Cell Formation and Neutralization by Human PRELP Antibody. Recombinant Human PRELP (Catalog # 6447-PR) inhibits Recombinant Mouse TRANCE induced osteoclast-like cell formation in the RAW 264.7 mouse monocyte/macrophage cell line in a dose-dependent manner (orange line), as measured by the TRAP (tartrate-resistant acid phosphatase) solution assay. Inhibition of Recombinant Mouse TRANCE (5 ng/mL) activity elicited by Recombinant Human PRELP (5 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human PRELP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6447). The ND50 is typically 0.2-1.2 µg/mL in the presence of Recombinant Mouse M-CSF (20 ng/mL).
