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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [3]
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- Product number
- NBP2-21658 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TIMP-4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. TIMP4 antibody is specific for human TIMP4 protein. This antibody reacts with human TIMP4 in tissue sections of formalin-fixed, paraffin-embedded In ELISAs and Native-PAGE, this antibody does not cross-react with recombinant human TIMP-1, -2 or -3.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Development of a monoclonal antibody that specifically detects tissue inhibitor of metalloproteinase-4 (TIMP-4) in formalin-fixed, paraffin-embedded human tissues.
Donover PS, Wojciechowski BS, Thirumaran R, Zemba-Palko V, Prendergast GC, Wallon UM
Journal of cellular biochemistry 2010 Aug 1;110(5):1255-61
Journal of cellular biochemistry 2010 Aug 1;110(5):1255-61
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TIMP-4 Antibody (9.4-7) [NBP2-21658] - Mouse Anti-TIMP-4 Antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. a) isotype specific IgG2bk monoclonal; b) TIMP-4 antibody ; and c) TIMP-4 each at 10 ug/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: TIMP-4 is secreted. Staining: TIMP-4 as precipitated brown signal.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TIMP-4 Antibody (9.4-7) [NBP2-21658] - Tissue: small intestine. Fixation: formalin fixed paraffin embedded. Primary antibody: anti-TIMP4 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: TIMP4 as precipitated red signal with hematoxylin purple nuclear counterstain.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TIMP-4 Antibody (9.4-7) [NBP2-21658] - Tissue: kidney. Fixation: formalin fixed paraffin embedded. Primary antibody: anti-TMIP4 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: TMIP4 as precipitated red signal with hematoxylin purple nuclear counterstain.
