Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A07131 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-TIMP4 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Metalloproteinase inhibitor 4(TIMP4) detection. Tested with WB, IHC-P, ICC, ICC/IF, FCM, ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Doxycycline Attenuates Doxorubicin-Induced Cardiotoxicity by Improving Myocardial Energy Metabolism in Rats.
Dantas D, Pereira AG, Fujimori ASS, Ribeiro APD, de Almeida Silva CCV, Monte MG, CorrĂȘa CR, Fernandes AA, Bazan SGZ, Azevedo PS, Minicucci MF, de Paiva SAR, Zornoff LAM, Polegato BF
Journal of cardiovascular development and disease 2022 Aug 8;9(8)
Journal of cardiovascular development and disease 2022 Aug 8;9(8)
No comments: Submit comment
Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of TIMP4 using anti- TIMP4 antibody (A07131). TIMP4 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- TIMP4 Antibody (A07131) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image