NB600-252

Ma P, Pan H, Montgomery RL, Olson EN, Schultz RM
Proceedings of the National Academy of Sciences of the United States of America 2012 Feb 21;109(8):E481-9

Mediator coordinates PIC assembly with recruitment of CHD1.

Lin JJ, Lehmann LW, Bonora G, Sridharan R, Vashisht AA, Tran N, Plath K, Wohlschlegel JA, Carey M
Genes & development 2011 Oct 15;25(20):2198-209

Combinatorial patterning of chromatin regulators uncovered by genome-wide location analysis in human cells.

Ram O, Goren A, Amit I, Shoresh N, Yosef N, Ernst J, Kellis M, Gymrek M, Issner R, Coyne M, Durham T, Zhang X, Donaghey J, Epstein CB, Regev A, Bernstein BE
Cell 2011 Dec 23;147(7):1628-39

Chromatin boundaries require functional collaboration between the hSET1 and NURF complexes.

Li X, Wang S, Li Y, Deng C, Steiner LA, Xiao H, Wu C, Bungert J, Gallagher PG, Felsenfeld G, Qiu Y, Huang S
Blood 2011 Aug 4;118(5):1386-94

Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger.

Wang GG, Song J, Wang Z, Dormann HL, Casadio F, Li H, Luo JL, Patel DJ, Allis CD
Nature 2009 Jun 11;459(7248):847-51

Epigenetic regulation of dendritic cell-derived interleukin-12 facilitates immunosuppression after a severe innate immune response.

Wen H, Dou Y, Hogaboam CM, Kunkel SL
Blood 2008 Feb 15;111(4):1797-804

The histone H3 lysine-27 demethylase Jmjd3 links inflammation to inhibition of polycomb-mediated gene silencing.

De Santa F, Totaro MG, Prosperini E, Notarbartolo S, Testa G, Natoli G
Cell 2007 Sep 21;130(6):1083-94

A histone H3 lysine 27 demethylase regulates animal posterior development.

Lan F, Bayliss PE, Rinn JL, Whetstine JR, Wang JK, Chen S, Iwase S, Alpatov R, Issaeva I, Canaani E, Roberts TM, Chang HY, Shi Y
Nature 2007 Oct 11;449(7163):689-94

CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation.

Higa LA, Wu M, Ye T, Kobayashi R, Sun H, Zhang H
Nature cell biology 2006 Nov;8(11):1277-83

Regulation of MLL1 H3K4 methyltransferase activity by its core components.

Dou Y, Milne TA, Ruthenburg AJ, Lee S, Lee JW, Verdine GL, Allis CD, Roeder RG
Nature structural & molecular biology 2006 Aug;13(8):713-9

WDR5 associates with histone H3 methylated at K4 and is essential for H3 K4 methylation and vertebrate development.

Wysocka J, Swigut T, Milne TA, Dou Y, Zhang X, Burlingame AL, Roeder RG, Brivanlou AH, Allis CD
Cell 2005 Jun 17;121(6):859-72

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: RbBP5 Antibody [NB600-252] - Whole cell lysate (50 ug for WB and Input; one 10 cm plate for IP) from 293T cells. Antibody used at the indicated concentrations for WB or 10 ug for IP (15% of IP loaded). Immunoprecipitated RbBP5 was detected by WB using 0.2 mg/ml.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: RbBP5 Antibody [NB600-252] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RbBP5 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: RbBP5 Antibody [NB600-252] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RbBP5 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: RbBP5 Antibody [NB600-252] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- RbBP5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Chromatin Immunoprecipitation: RbBP5 Antibody [NB600-252] - Chromatin Immunoprecipitation: RbBP5 Antibody [NB600-252] - ChIP-chip scatter plot of anti-RbBP5 enriched DNA binding sites versus input reference DNA. A. 10 ug of NB600-252 was used to immunoprecipitate chromatin from K562 cells per Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-RbBP5 ChIP, normal rabbit IgG showed little enrichment.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Chromatin Immunoprecipitation Sequencing: RbBP5 Antibody [NB600-252] - Localization of RbBP5 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-RbBP5 antibody NB600-252 and analyzed by DNA sequencing. The figure illustrates the peak distribution of RbBP5 binding within a 500 Kb region of chromosome 1 as detected using anti-RbBP5 antibody NB600-252. ChIP-seq validation performed by Diogenode, Denville, NJ.