PAB3168

antibody from Abnova Corporation

Targeting: STK25 SOK1, YSK1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3168

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3168, RRID:AB_1581067

Product name

STK25 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of STK25.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

YSK1, a novel mammalian protein kinase structurally related to Ste20 and SPS1, but is not involved in the known MAPK pathways.

Osada S, Izawa M, Saito R, Mizuno K, Suzuki A, Hirai S, Ohno S
Oncogene 1997 May 1;14(17):2047-57

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Activation of a human Ste20-like kinase by oxidant stress defines a novel stress response pathway.

Pombo CM, Bonventre JV, Molnar A, Kyriakis J, Force T
The EMBO journal 1996 Sep 2;15(17):4537-46

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of STK25 polyclonal antibody (Cat # PAB3168) in A-549 cell lysate. STK25 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with STK25 polyclonal antibody (Cat # PAB3168) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)