PA5-98758
antibody from Invitrogen Antibodies
Targeting: MFSD4A
DKFZp761N1114, FLJ25004, FLJ34577, MFSD4, UNQ3064
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-98758 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MFSD4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 2.53 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MFSD4 using a MFSD4 Polyclonal antibody (Product # PA5-98758) at a concentration of 3.38 µg/mL. Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 47 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MFSD4 using a MFSD4 Polyclonal antibody (Product # PA5-98758) at a concentration of 3.38 µg/mL. Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 47 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MFSD4 in Hela cells using a MFSD4 polyclonal antibody (Product # PA5-98758) at a dilution of 1:166. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.