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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 12023-2-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NUCKS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSRPVRNRK VVDYSQFQES DDADEDYGRD SGPPTKKIRS SPREAKNKRR SGKNSQEDSE DSEDKDVKTK KDDSHSAEDS EDEKEDHKNV RQQRQAASKA ASKQREMLME DVGSEEEQEE EDEAPFQEKD SGSDEDFLME DDDDSDYGSS KKKNKKMVKK SKPERKEKKM PKPRLKATVT PSPVKGKGKV GRPTASKASK EKTPSPKEED EEPESPPEKK TSTSPPPEKS GDEGSEDEAP SGED (1-243 aa encoded by BC000805)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.44 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12023-2-AP (NUCKS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 12023-2-AP (NUCKS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 12023-2-AP ( NUCKS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 12023-2-AP (NUCKS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12023-2-AP (NUCKS1 antibody) at dilution of 1:100 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12023-2-AP ( NUCKS1 antibody) at dilution of 1:100 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-NUCKS1 (IP:12023-2-AP, 4ug; Detection:12023-2-AP 1:500) with HEK-293 cells lysate 2800ug.
