Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-26535 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NUCKS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references CircRNA circ_0008037 facilitates tumor growth and the Warburg effect via upregulating NUCKS1 by binding to miR-433-3p in non-small cell lung cancer.
Yu J, Zhang H, Zhao C, Li G, Zhang Y, Sun Y
Thoracic cancer 2022 Jan;13(2):162-172
Thoracic cancer 2022 Jan;13(2):162-172
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a NUCKS1 polyclonal antibody (Product # PA5-26535) in Ramos cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Ramos cells using a NUCKS1 polyclonal antibody (Product # PA5-26535) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Ramos cells using a NUCKS1 polyclonal antibody (Product # PA5-26535) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 5 NUCKS1 acted as a target of miR-433-3p. (a) The alignment of miR-433-3p with the 3'UTR of NUCKS1. (b, c) Dual-luciferase reporter assay was executed to analyze the luciferase intensity of the psiCHECK-2 vector carrying NUCKS1 3'-UTR-WT or NUCKS1 3'-UTR-MUT in NSCLC cells transfected with miR-NC or miR-433-3p mimic. (d, e) RT-qPCR and western blotting were executed to analyze the levels of NUCKS1 mRNA and protein in NSCLC cells transfected with miR-NC or miR-433-3p mimic. (f, g) RT-qPCR and western blotting were carried out to assess the levels of NUCKS1 mRNA and protein in NSCLC tissues and paracarcinoma tissues. (h) Correlation of NUCKS1 mRNA and miR-433-3p in NSCLC tissues was analyzed by Pearson's correlation analysis. (i, j) RT-qPCR and western blotting were performed to examine the levels of NUCKS1 mRNA and protein in NSCLC cells and BEAS-2B cells. (k, l) Levels of NUCKS1 mRNA and protein in NSCLC cells transfected with si-NC, si-circ_0008037, si- circ_0008037 + anti-miR-NC, or si-circ_0008037 + anti-miR-433-3p were detected by RT-qPCR and western blotting. (m) Correlation of NUCKS1 mRNA and circ_0008037 in NSCLC tissues was analyzed by Pearson's correlation analysis. * P < 0.05