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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- 600-101-285 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-101-285, RRID:AB_2265003
- Product name
- Anti-p28 ING5 (GOAT) Antibody - 600-101-285
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references The inhibitor of growth protein 5 (ING5) depends on INCA1 as a co-factor for its antiproliferative effects.
Zhang F, Bäumer N, Rode M, Ji P, Zhang T, Berdel WE, Müller-Tidow C
PloS one 2011;6(7):e21505
PloS one 2011;6(7):e21505
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot analysis is shown using Rockland's Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification is shown in lane 1. Comparison to a molecular weight marker (not shown) indicates a single band of ~45.0 kDa corresponding to the expected molecular weight for the recombinant protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using chemiluminescence reagent with a 5-min exposure time. Other detection systems will yield similar results. Personal communication Jacques Cote.
- Validation comment
- SDS PAGE
