13895-1-AP

D2HGDH antibody from Proteintech Group

D2HGD, FLJ42195, MGC25181

Western blot

Supportive data in Antibodypedia

ELISA

Data presented on provider website

Immunocytochemistry

Data presented on provider website

Immunoprecipitation

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for 13895-1-AP

Antibody Data

Product number

13895-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#13895-1-AP, RRID:AB_2089330

Product name

D2HGDH antibody

Provider product page

Antibody type

Polyclonal

Description

KD/KO validated D2HGDH antibody (Cat. #13895-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

D-2-hydroxyglutarate dehydrogenase in breast carcinoma as a potent prognostic marker associated with proliferation.
Hayashi C, Takagi K, Sato A, Yamaguchi M, Minemura H, Miki Y, Harada-Shoji N, Miyashita M, Sasano H, Suzuki T
Histology and histopathology 2021 Oct;36(10):1053-1062

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Urinary 2-Hydroxyglutarate Enantiomers Are Markedly Elevated in a Murine Model of Type 2 Diabetic Kidney Disease.
Baek J, Pennathur S
Metabolites 2021 Jul 21;11(8)

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MYC Regulation of D2HGDH and L2HGDH Influences the Epigenome and Epitranscriptome.
Qiu Z, Lin AP, Jiang S, Elkashef SM, Myers J, Srikantan S, Sasi B, Cao JZ, Godley LA, Rakheja D, Lyu Y, Zheng S, Madesh M, Shiio Y, Dahia PLM, Aguiar RCT
Cell chemical biology 2020 May 21;27(5):538-550.e7

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Degradation of D-2-hydroxyglutarate in the presence of isocitrate dehydrogenase mutations.
Berger RS, Ellmann L, Reinders J, Kreutz M, Stempfl T, Oefner PJ, Dettmer K
Scientific reports 2019 May 15;9(1):7436

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D-2-hydroxyglutaric aciduria Type I: Functional analysis of D2HGDH missense variants.
Pop A, Struys EA, Jansen EEW, Fernandez MR, Kanhai WA, van Dooren SJM, Ozturk S, van Oostendorp J, Lennertz P, Kranendijk M, van der Knaap MS, Gibson KM, van Schaftingen E, Salomons GS
Human mutation 2019 Jul;40(7):975-982

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D2HGDH regulates alpha-ketoglutarate levels and dioxygenase function by modulating IDH2.
Lin AP, Abbas S, Kim SW, Ortega M, Bouamar H, Escobedo Y, Varadarajan P, Qin Y, Sudderth J, Schulz E, Deutsch A, Mohan S, Ulz P, Neumeister P, Rakheja D, Gao X, Hinck A, Weintraub ST, DeBerardinis RJ, Sill H, Dahia PL, Aguiar RC
Nature communications 2015 Jul 16;6:7768

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Hypoxia Induces Production of L-2-Hydroxyglutarate.
Intlekofer AM, Dematteo RG, Venneti S, Finley LW, Lu C, Judkins AR, Rustenburg AS, Grinaway PB, Chodera JD, Cross JR, Thompson CB
Cell metabolism 2015 Aug 4;22(2):304-11

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Hypoxia-Mediated Increases in L-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress.
Oldham WM, Clish CB, Yang Y, Loscalzo J
Cell metabolism 2015 Aug 4;22(2):291-303

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D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth.
Ma S, Jiang B, Deng W, Gu ZK, Wu FZ, Li T, Xia Y, Yang H, Ye D, Xiong Y, Guan KL
Oncotarget 2015 Apr 20;6(11):8606-20

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Screen for IDH1, IDH2, IDH3, D2HGDH and L2HGDH mutations in glioblastoma.
Krell D, Assoku M, Galloway M, Mulholland P, Tomlinson I, Bardella C
PloS one 2011;6(5):e19868

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human liver tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP(D2HGDH antibody) at dilution of 1:500

Sample type

tissue

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP result of anti-D2HGDH (13895-1-AP for IP and Detection) with mouse liver tissue lysate.

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-D2HGDH (IP:13895-1-AP, 3ug; Detection:13895-1-AP 1:500) with mouse liver tissue lysate 5000ug.

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver cancer using 13895-1-AP(D2HGDH antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The D2HGDH antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human D2HGDH. This antibody recognizes human,mouse,rat antigen. The D2HGDH antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.

Appendix

13895-1-AP.pdf

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