Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [2]
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Validation data
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- Product number
- 13895-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- D2HGDH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MLPRRPLAW PAWLLRGAPG AAGSWGRPVG PLARRGCCSA PGTPEVPLTR ERYPVQRLPF STVSKQDLAA FERIVPGGVV TDPEALQAPN VDWLRTLRGC SKVLLRPRTS EEVSHILRHC HERNLAVNPQ GGNTGMVGGS VPVFDEIILS TARMNRVLSF HSVSGILVCQ AGCVLEELSR YVEERDFIMP LDLGAKGSCH IGGNVATNAG GLRFLRYGSL HGTVLGLEVV LADGTVLDCL TSLRKDNTGY DLKQLFIGSE GTLGIITTVS ILCPPKPRAV NVAFLGC (1-286 aa encoded by BC036604)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human liver tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat liver tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 13895-1-AP (D2HGDH antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 13895-1-AP ( D2HGDH Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 13895-1-AP ( D2HGDH Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-D2HGDH (13895-1-AP for IP and Detection) with mouse liver tissue lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-D2HGDH (IP:13895-1-AP, 3ug; Detection:13895-1-AP 1:500) with mouse liver tissue lysate 5000ug.