66364-1-IG

D2HGDH antibody from Invitrogen Antibodies

D2HGD, FLJ42195, MGC25181

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for 66364-1-IG

Antibody Data

Product number

66364-1-IG

Provider

Invitrogen Antibodies

Product name

D2HGDH Monoclonal Antibody (1E9B3)

Provider product page

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: MLPRRPLAW PAWLLRGAPG AAGSWGRPVG PLARRGCCSA PGTPEVPLTR ERYPVQRLPF STVSKQDLAA FERIVPGGVV TDPEALQAPN VDWLRTLRGC SKVLLRPRTS EEVSHILRHC HERNLAVNPQ GGNTGMVGGS VPVFDEIILS TARMNRVLSF HSVSGILVCQ AGCVLEELSR YVEERDFIMP LDLGAKGSCH IGGNVATNAG GLRFLRYGSL HGTVLGLEVV LADGTVLDCL TSLRKDNTGY DLKQLFIGSE GTLGIITTVS ILCPPKPRAV NVAFLGC (1-286 aa encoded by BC036604)

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

1E9B3

Vial size

150 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66364-1-IG ( D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using D2HGDH antibody (Product # 66364-1-IG, Clone: 1E9B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66364-1-IG ( D2HGDH Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66364-1-IG ( D2HGDH Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).